A
total
of
201,
218,
258,
253,
and
244
polymorphic
AFLP
and
RAPD
markers
were
9501,
9502,
55
-
3
x
8380
-
1,
8380
-
1
x
55
-
3,
and
55
-
1
x
8380
-
1,
respectively
.
each
each
in
ea
ic
RAPD
loci
were
scored
for
each
RAPD
8380
-
1
x
55
-
integ
3
x
8
d
55
-
1
x
8380
-
1
populations,
focused
on
markers
exhibiting
conta
1)
.
M
welve
different
map
locations,
chac
ed
clustering
of
Eco
RI
+
Mse
I
AFLP
map
nnellii
and
suggested
that
regio
ndom
distribution
of
markers
on
the
from
were
mark
potat
popu
arity
over
the
3'
end
to
a
region
n
Arabidopsis
thaliana
chromosome
1
(BAC
F3F20
.
1;
annotated
as
callose
synthase)
.
This
region
may
be
a
key
site
for
determination
of
solanidine
in
Solanum
spp
.
,
solanidine
or
solasodine
in
S
.
tuberosum
x
S
.
berthaultii
progeny
(Yencho
et
al
.
1998),
and
for
accumulation
of
solanidine
or
leptinidine
in
S
.
chacoense
(Ronning
et
al
.
1999)
.
Marker
UBC370
-
1500
exhibited
linkage
to
a
number
of
AFLP
and
RAPD
markers
in
linkage
group
1
marker
frameworks
for
55
-
3x8380
-
1/8380
-
1x55
-
3
and
9501/9502
populations
.
This
marker
was
also
detected
in
the
high
-
leptine
genotype
8380
-
1
.
As
a
result,
we
would
expect
no
correlation
between
presence
of
the
marker
and
leptine
content
in
the
55
-
3x8380
-
1/8380
-
1x55
-
3
and
55
-
1x8380
-
1
families
.
These
results,
together
with
previous
genetic
studies
involving
crosses
with
8380
-
1
(Ronning
et
al
.
,
1998),
suggest
that
additional
genetic
factors
likely
influence
the
inheritance
and
expression
of
leptine
glycoalkaloids
in
S
.
chacoense
.
The
markers
mapped
here
provide
an
opportunity
for
additional
fine
mapping
of
this
region
on
chromosome
1
and
investigation
of
glycoalkaloid
inheritance
.
scored
in
families
These
markers
were
primarily
AFLP
loci,
but
also
included
an
average
of
38
RAPD
loci
in
of
the
five
families
.
An
average
of
17
polymorphic
loci
(range
9
-
33)
were
scored
for
AFLP
primer
pair
.
An
average
of
39
AFLP
loci
were
monomorphic
across
all
individuals
ch
family
.
One
to
nine
(mean
3
.
6)
polymorph
primer
in
these
families
.
Unambiguous
mapped
markers
in
families
9501,
9502,
3,
55
-
3
x
8380
-
1,
and
55
-
1
x
8380
-
1
totaled
158,
141,
180,
185,
and
118,
respectively
.
The
rated
map
for
chromosome
1,
derived
from
merging
linkage
groups
from
9501/9502,
55
-
380
-
1/8380
-
1
x
55
-
3,
an
linkage
with
chromosome
1
marker
UBC370
-
1500
and
allied
linkage
groups
.
This
map
ined
six
RAPD
markers
and
45
AFLP
markers
and
spanned
77
.
9
centimorgans
(Figure
arkers
exhibiting
identical
segregation
are
evident
at
t
with
each
locus
containing
two
to
four
markers
.
Dense
clustering
of
Eco
RI
+
Mse
I
AFLP
markers
occurred
in
linkage
groups
of
all
S
.
oense
populations
.
Haanstra
et
al
.
(1999)
observ
markers,
but
not
Pst
I
+
Mse
I
AFLP
markers,
in
centromeric
regions
on
all
chromosomes
of
a
constructed
from
a
cross
of
Lycopersicon
esculentum
x
L
.
pe
the
clustering
of
markers
was
due
to
a
suppression
of
recombination
in
the
heterochromatic
ns
near
the
centromeres,
rather
than
to
a
non
-
ra
chromosomes
.
Using
6
AFLP
primer
combinations
and
a
mapping
population
generated
a
cross
of
non
-
inbred
potato
parents,
Van
Eck
et
al
.
(1995)
found
that
AFLP
markers
generally
randomly
distributed,
but
also
observed
clustering
of
Eco
RI
+
Mse
I
-
based
ers
.
The
RAPD
marker
UBC370
-
1500,
previously
mapped
to
the
top
of
chromosome
1
of
o
and
tomato
and
shown
to
be
linked
to
nil
leptine
production
in
the
9501/9502
lations
of
S
.
chacoense
(Ronning
et
al
.
,
1999),
has
simil
o
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