An
AFLP
Marker
-
Based
Linkage
Map
of
Solanum
chacoense
Bitter
Chromosome
1
Catherine
M
.
Ronning
1
,
Kenneth
M
.
Haymes
2
,
Lind
L
.
Sanford
1
and
John
R
.
Stommel
1
USDA,
ARS,
Vegetable
Laboratory
1
,
Fruit
Laboratory
2
,
10300
Baltimore
Ave
.
,
Beltsville,
MD
20705
Present
addresses:
C
.
M
.
Ronning,
The
Institute
for
Genomic
Research,
9712
Medical
Center
Dr
.
,
Rockville,
MD
20850;
K
.
Haymes,
D2
Biotechnologies
Incorporated,
P
.
O
.
Box
78843,
Atlanta,
GA
30314;
L
.
L
.
Sanford,
retired
.
Introduction
Solanum
chacoense
Bitter,
the
Chaco
potato,
is
a
wild
species
related
to
the
cultivated
potato,
S
.
tuberosum
L
.
Solanum
chacoense
is
diploid,
tuberous,
and
self
-
incompatible,
and
is
indigenous
to
northern
Argentina
and
the
surrounding
areas
.
The
species
is
of
interest
to
plant
breeders
because
some
individuals
produce
leptine
glycoalkaloids,
which
are
believed
to
be
factors
in
the
resistance
of
S
.
chacoense
to
the
Colorado
potato
beetle
(CPB;
Leptinotarsa
decemlineata
Say)
(Kuhn
and
Löw,
1961)
.
The
leptines
are
a
unique
class
of
glycoalkaloids
whose
production
and
accumulation
is
foliar
-
specific
(Kuhn
and
Löw,
1961;
Sinden
et
al
.
,
1986b)
.
Since
resistance
-
conferring
glycoalkaloids
are
toxic
to
humans
and
accumulate
in
tubers,
foliar
-
specific
leptines
offer
an
attractive
opportunity
to
develop
useful
CPB
host
-
resistance
in
S
.
tuberosum,
and
perhaps
in
related
species
.
In
reciprocal
F
1
crosses
and
backcrosses
between
high
-
leptine
producing
S
.
chacoense
genotypes
and
nil
-
leptine
genotypes,
leptine
content
exhibited
discrete
distributions
in
most
families
with
high
an
d
low
classes
of
leptine
-
producing
individuals,
suggestive
of
genetic
control
by
a
single
recessive
gene
.
However,
a
number
of
families
produced
from
crosses
of
high
x
low
leptine
-
producers
and
low
x
low
leptine
-
producers
exhibited
continuous
distributions
for
leptine
content,
suggesting
the
presence
of
additional
genetic
factors
that
affect
the
inheritance
of
leptine
glycoalkaloids
in
these
S
.
chacoense
populations
(Ronning
et
al
.
,
1998)
.
A
RAPD
marker,
UBC370
-
1500,
was
identified
in
selected
reciprocal
F
1
families
as
being
tightly
linked
to
nil/low
leptine
content
(Ronning
et
al
.
,
1999)
.
RAPD
marker
UBC370
-
1500
was
mapped
to
chromosome
1
of
potato
and
tomato
(Ronning
et
al
.
,
1999),
near
the
site
of
a
major
QTL
for
solanidine
accumulation
in
S
.
tuberosum
x
S
.
berthaultii
(Yencho
et
al
.
,
1998),
suggesting
that
this
region
on
chromosome
1
may
be
important
in
glycoalkaloid
production
.
In
this
paper,
we
report
results
contributing
to
the
construction
of
a
marker
-
based
linkage
map
of
S
.
chacoense
chromosome
1
using
primarily
AFLPs,
plus
a
number
of
RAPD
markers
.
The
marker
UBC370
-
1500
was
used
as
an
“anchor”
to
coordinate
linkage
data
from
five
related
F
1
families,
derived
from
crosses
between
high
x
low
and
high
x
high
leptine
-
producing
lines
that
originated
from
three
different
S
.
chacoense
accessions,
in
the
construction
of
an
integrated
linkage
map
for
this
chromosome
.
The
results
presented
are
intended
for
further
use
by
programs
active
in
glycoalkaloids
and
marker
-
based
research
in
Solanaceous
species
.
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