Figure
1
.
Segregation
of
192
F
2
plants
for
the
PCR
marker
91SP6
.
Leaf
disks
of
the
seedlings
were
placed
into
96
-
well
microtiter
plates
and
DNA
was
isolated
according
method
described
here
.
The
DNA
was
used
in
a
PCR
and
the
products
were
loa
1
.
5%
agarose
gel
.
The
gel
was
run
at
80
V
for
45
min
and
then
stained
in
10
mg/ml
ethidium
bromide
.
to
the
ded
to
a
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