Solutions : - Grinding buffer : A mix of   extraction buffer, nuclei lysis buffer   and 5% sarcosyl in a 10:10:4 volume ratio . -   - Extraction buffer (1 liter):                64 g                  Sorbitol                                                              12 g                Trizma base                                                              1 . 7 g                EDTA Bring to pH 7 . 5 using concentrated HCl, keep at 4 ° C . Before use add 3 . 8 g of sodium bisulfite . - Nuclei lysis buffer (1 liter):               20 g                    CTAB                                                               200 ml               1 M Tris pH 7 . 5                                                                200 ml                0 . 25 M EDTA Mix all of the above . After CTAB dissolves completely, add 400 ml of 5M NaCl . - Bring to pH 7 . 5 using concentrated HCl 5% Sarcosyl (1 liter):                      50 g                 N - Lauroylsarcosine - - Storage buffer (1 liter):                      800 ml             95% Ethanol                                                                100 ml          2 M Sodium Acetate         Bring to pH 7 . 0 using concentrated 5 M NaOH . - TE; Tris EDTA buffer (100 ml)         1 ml            1 M Tris pH 8 . 0                                                                 40 m l                0 . 25 M EDTA        Bring to pH 8 . 0 using 5 M NaOH . Figure 1 demonstrates the segregation of 192 tomato plants for the PCR marker 91SP6 . The plants were sown in 16 x 8 (columns x rows) trays and leaf disks from the seedlings were placed in the 96 microtiter plate for DNA isolation . All plants were sampled, extracted and genotyped on the same day . The genotype of the plants was verified using a conventional RFLP analysis with the PCR product . The time spent on extracting the DNA from 384 leaf samples (four plates) was only 2 h, for one person . This technique enables the genotyping of a large population in a short time to identify rare genetic events .   References 1 . Deragon, J . M . and Landry, B . S . (1992) PCR Methods Appl . 3, 175 - 180 . 2 . Geuna, F . , Hartings, H . and Scienza, A . (2000) Anal . Biochem . 278, 228 - 230 . 3 . Wang, H . , Qi, M . and Cutler, A . J . (1993) Nucleic Acids Res . 21, 4153 - 4154 .

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