Chromosome location of tomato ESTs related to carbon metabolism ) MR Physiologie et Biotechnologie Végétales IBVI - INRA   BP 81 - 33 883 Villenave d'Ornon - Cedex (France) I didate genes linked to sugar/acid metabolism content in to n h tomato EST database (h ww . he a h a u the p o . el L . esculentum w the genome to be segmented (Pan et a . n was connected to the high - density map of tomato (Tanksley et al 99 ) by pro ing all the ILs wi e m ening f ing four restriction enzymes (EcoRI, EcoRV, Hind III, and XbaI) . Genomic DNA extraction, digestion, b re as d i mbani et ) w ls e n an Colombani et al, 2000) and their l was ro m y e m m d hey ted ly , h metabolism, ement as te g related to e d References Es sion line population of Lycopersicon pennellii in the ted s the id ld . cs Liu n O , Z D ) rati nucleo ene d a A p 309 - 322 ma e o e 43: 29 - 40 SD, Ganal MW, Prince JP n r u , nno r e R ler L, Paterson AH, Pinedo O, Roder MS, Wing RA, Wu W, Young ND (1992) High density molecular linkage of t ge 1 P . Duffe (1), M . C . Gomez (1), D . Just (2), M . Lemaire - Chamley (2), C . Rothan (2), and M . Causse (1) (1) INRA Unité de Génétique et Amelioration des Fruits et Légumes - BP 94 - 84143 ontfavet - Cedex (France) M (2 U n order to screen for putative can fru 48 mato it, we selected EST clo es in t e TIGR ttp://w tigr . org/tdb/tgi/lgi/) . T se clones were loc ted on t e tom to map sing opulation f introgression lines (ILs) having one segment of L penn lii (LA716) in a (M82) background (Eshed and Zamir, 1995) . The 75 ILs allo into 107 bins l, 2000; http://www . sgn . cornell . edu/) The IL populatio , 1 2 b th the RFLP markers from the framework F2 map (Pan et al, 2000) . The ESTs wer apped by RFLP after scre or polymorphism us and hy ridization we escribed n Saliba - Colo al (2000 . A few ESTs ere a o mapp d i intraspecific population (Saliba - ocation in the IL map deduced f m the common RFLP arkers . All fourt ight ESTs involved in carbon metabolis were appe (Table 1) . T represen enzymes involved in transport and a few oth Calvin cyc er functions . They revealed 57 le, glyco sis, TCA cycle loci . Their involv sugar and starc candida enes for QTLs carbon m tabolism remains to be stu ied . hed Y, Zamir D (1995) An introgres cultiva tomato enable entification and fine mapping of yie - associated QTLs Geneti 141: 1147 - 1162 Pan Q, Y - S, Budai - Hadria , Sela M Carmel - Goren L, amir , Fluhr R . (2000 Co homologues in the genomes of two dicotyle mpa ve genetics of tide binding site - leu ons: tom cine rich to and repea rabido t n resista sis . Genetics 155: ce g Saliba - Colombani V, Causse M, Gervais L, Philouze J (2 m 000) Efficiency of AFLP, RAPD and RFLP rkers for the construction of an intraspecific ap of th tomat genome . Genom Tanksley , de Vice te MC, Bonierbale MW, B oun P, F lton TM Giova ni JJ, Grandillo S, Ma tin GB, M sseguer , Miller JC, Mil maps he tomato and potato nomes . Genetics 132: 1141 - 1 60

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