Comparing the performance of a set of processing tomato lines nearly isogenic for the ms-1035 gene for male-
sterility
Tanksley, S.1, Bernacchi, D.1, Fulton, T.1, Beck-Bunn, T.2, Emmatty, D.3, Eshed, Y.4, Inai, S.5, Lopez, J6 ,
Petiard, V.7 , Sayama, H.5, Uhlig, J.2,
& Zamir, D.4
1 Department of Plant Breeding and Biometry, Cornell University, Ithaca, NY 14853
2 Seminis Vegetable Seeds, Woodland, CA 95695, U.S.A.
3 Heinz U.S.A., Stockton, CA 95201
4 Hebrew University,Rehovot 76-100, Israel
5 Nippon Del Monte,Numata, Gumma 378 Japan
6 Nestle R & D Center S. A., Apartado 435, E-05080, Badajoz, Spain
7 Centre Recherche Nestle, 101 av. G. Eiffel, 37390 NOTRE DAME D'OE, Tours Cedex, France
Gene ms-10 is one of a number of nuclear male sterile genes reported in tomato which can be used in the
production of F1 hybrid seeds. This gene is on chromosome 2 and is tightly linked to the morphological marker aa
(anthocyaninless) and the isozyme marker Prx-2. A fresh market stock has been produced in which the recessive
ms-1035 is in cis with the recessive aa allele and a rare allele of the peroxidase locus, Prx-21. The peroxidase
allele and the anthocyanin allele provide selectable markers for backcross and seedling selection of the recessive
ms-10 allele (Tanksley and Zamir 1988).
We wanted to test the potential effects of ms-1035, and its associated DNA containing aa and Prx-21, on
the yield and quality of processing tomatoes to determine the feasibility of using this gene for producing F1,
hybrids. We therefore produced two nearly isogenic lines (NIL) for the ms-1035 gene in the genetic background of
the open pollinated, processing variety E6203. To construct these NILs, the Prx-21--ms-1035--aa cluster was
backcrossed 6 times, using marker assisted selection, into E6203 and selfed in the last generation to produce
homozygous male sterile plants. The source of the Prx-21-ms-1035--aa cluster was an Israeli processing breeding
line into which the cluster had been transferred from the original fresh market source described in Tanksley and
Zamir (1988). Two separate BC6S1 families were selected from which male-steriles could be identified in the
segregating generation. We refer to these lines as 95T1293 and 95T1184. Male steriles from each line were
hybridized to the recurrent parent, E6203, to generate two heterozygous, fertile seed batches for field testing.
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