Recombinations of lateral suppressor, ls, with marker genes.

Williams, W.

The lateral suppressor gene was reported in TGC5:6. The Following ratios are of F2 progeny segregating from repulsion.

       Chromo-
       some or             +       ls      X^2            X^2
Tester Linkage ++  + ls  tester  tester  tester  X^2 ls  Linkage
________________________________________________________________
 al       8   562   172   190      68     0.538  0.344   0.867
 f        V   565   165   187      75     1.054  0.344   3.792
 wt       7   585   183   167      57     3.096  0.344   0.258
 dm       IX  524   181   228      59     8.177  0.344   3.316
 j\1      V   561   178   191      62     0.134  0.344   0.016
 l\1      8   542   208   190      45     0.685  0.247   6.882
 c        IV  538   195   194      58     0.179  0.247   1.291
 d        2   569   201   163      52     5.29           0.341
 y        1   546   170   184      79     1.814  0.098   4.316
 y        1   424   143   164      60     4.646  0.186   0.259
 r        II  568   180   162      69     1.029  0.098   3.015
 t        10  440   152   148      51     0.011  0.186   0.0001
 mc       7   438   165   167      47     0.621          2.479
 e        XI  443   153   162      5      1.83   0.392   0.114
 u        10  471   179   134      33     9.065          3.977
              _______________________  

 a        V   436   173   169      39     0.092  0.392   7.768
 a)           550   183   182      70     0.179  0.247   0.815
              __________________________________________________
   Total a    986   356   351     109     0.267  0.622   1.462
Conclusive evidence on the linkage relationships of "ls" cannot be derived from the above figures. Although significant X^2 was obtained in one of the two families involving anthocyaninless (-a linkage group V), the independent segregations of ls - j\1 and ls - f make it unlikely that ls is a member of this linkage group. Further tests involving other markers on chromosome 8, such as "dl" and "bu" might prove fruitful.

One family provided information on the relationships between j and wt. The F progeny gave the following ratio:

         

+ +   + j   wt +   j wt   X^2 j   X^2 wt   X^2 Linkage

582   186   157     67    0.134   3.096     2.725
These data are consistent with the results of Rick and Barton from which it was concluded that j and wt are independent.