Segregation and linkage of solanifolia, sf, a new seedling marker.

Rick, C. M.

Solanifolia is a pleiotropic mutant picked up as an unfruitful mutant in a field of Pearson. As a seedling it shows considerable resemblance to c, but differs to such an extent in mature-plant characters that it should not be construed as a mimic and as the basis for a series of c-subscript symbols. The first true leaf usually has a large entire segment with a small basal lateral segment. This tendency to have a reduced number of segments continues throughout seedling development, but is never so extreme as in c or e. Margins of all leaves are entire. The interactions with c and e in the seedling stage are complex, but can be worked out. The compound sf-c, for example, is more reduced than either mutant alone, has single linear segments, but with one or two tiny basal segments, for its first five true leaves.

As a mature plant sf has about the same size and habit as normal Pearson, but its peculiar leaves give it a distinct appearance. They have narrow entire segments, of normal number, but are strongly folded and darker green. The smaller leaf segments are lacking. Flowers are also drastically modified: calyx reduced, corolla segments narrow, stamens mostly dialytic, pistil variable but usually split into separate carpels, often highly deformed. A few fruits set without aid, usually by the least deformed flowers. Fertility after artificial selfing and crossing is reasonably good.

Segregation data for 6 F2 families from different crosses reveals a very good fit to 3:1 (1688+:555 sf) with no significant departure from homogeneity (X^2 9.78 p=0.1-0.05). The following data show a linkage of 34 units between rv and sf, finally providing a long-sought neighbor to the former gene.

                                     Contingency
Tester     + +   + t   sf +   sf +   chi square        co.
________________________________________________________________
 a         110    37    26     10        0.01
 a         219    69    86     30        0.07
 ah        169    41    33     19        5.91
 c         101    46    25     11        0.01
 d         118    29    28      8        0.009
 dl        177    33    43      9        0.005
 l         238    50   100     16        0.53
 rv        164    49    80      7        8.15      33.0 +- 3.4
 rv        599    85   231     11       11.2       35.0 +- 1.9
 tf         65    26    21      7        0.016
 Wo         34    57     7     21        0.95
 Xa         26    65    11     17        0.70
 yv        161    49    44      8        1.12