Linkage tests with Cu.

Rick, C. M.

Dr. Young's Curly (Cu) mutant has been used in a series of exploratory linkage tests. With us it has performed as a very satisfactory seedling mutant. No clue for the phenotypic separation of Cu/Cu from Cu/+ has been found, but a diligent search in this direction would still be well justified. In six F2's of different crosses there was a consistent although slight deficiency of Cu phenotype, significant for the totals (414+:1089Cu. X^2=5.05, p=0.05- -0.02). The chi square for heterogenecity is not significant (6.59, p = 0.2- 0.1). Interactions with other seedling characters gave no trouble except for c and e. Among the Cu segregants it is very difficult to distinguish segregation for either gene; but very useful linkage information can still be gathered from the segregation among Cu^+ segregants.

The significant indications of linkage between Cu and d in the following data leave little doubt that Cu must abide in the crowded accomodations of chromosome 2. The mean of the two estimates of the d-Cu distance is 37 units, placing Cu beyond Wo, presumably close to Me.


                                      Contingency
tester   + +   + t    Cu +   Cu t     chi square      co.
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 a        52    20    172    42          1.66
 a        25     5     48    19          1.49
 ah       42    11    101    40          0.79
 sl       45    19     99    32          0.37
 c        58    14     --    --          0.91
 d        41    31    160    54          7.13      39.0 +- 2.6
 d        71    67    311   101         57.13      35.5 +- 2.5
 dl       37    16     99    42          0.01
 e        21     9     54    13          1.25
 H        54    10     98    33          1.76
 l\1      45    27    157    57          2.53
 La        4     8     18    45         '0.01
 rv       43    21     90    41          0.37
 Xa       23    34     42    82          4.60
 yv       38    15    111    30          0.38