Linkages of ten male-sterile genes.<p>

Pratt, D.  <p>

Ten mutants from the San Marzano variety -- ms\3, ms\5, ms\6, ms\7, ms\8,
ms\9, ms\11, ms\12, ms\13, ms\15 -- were tested against the following marker
genes from five stocs: d, r, y, c,l\1; ah, yv, dl; Wo^m, tf; e, a, wf, mc; Xa.
The current, linkage map show that one or more of these markers appears in
each of the ten known groups of linked genes.<p>
     
The tests revealed linkages for eight of the ten ms genes.  Another research
note outlines the technique used in detecting these linkages.  The maximum
likelihood method was used to estimate the recombination values and their
standard errors.<p>
<pre>

ms    marker   linkage      chromosome   linkage group

ms\3    a      .27 +- .02      -              V
ms\5    d      .13 +- .04      2              I
ms\6    y      .30 +- .07      1              III     
ms\7    a      .15 +- .02      -              V
ms\8    dl     .00 +- .05      8              VIII                              
ms\8    l\1    .15 +- .02      8              VIII
ms\9    wf     .20 +- .02      3              II                    
ms\9    r      .21 +- .08      3              II                     
ms\12   a      .13 +- .02      -              V                  
ms\15   d      .08 +- .02      2              I                 
ms\15   Wo^m   .09 +- .03      2              I                  
</pre>

None of the demonstrated linkages to good seedling markers appears tight
enough for practical use in the maintemnce of an ms parent line for hybrid
seed production (using the linked marker to identify ms segregants in the
seedling stage).  The tests did show that both ms\5, and ms\15 could lie close
to the anthocyaninless gene aw on chromosome 2. This particular marker was not
used in the linkage tests but should serve well for ms line maintenance if
closely linked to either ms mutant.<p>
<!--GH_SEARCH-->
<!--GH_END-->