Chiscon, J. A.
A 25% sucrose solution containing 30 ppm boron as boric acid crystals has been used as a dependable medium for germination of pollen from line 206 of L. pimpinellifoliwn. During a series of experiments, freshly collected pollen was dusted with a brush to the bottom of well slides. A drop of the above solution was then placed on the pollen. A lubricant was applied to the outer rim of the wells and the wells were then sealed with a cover slip. Kept at room temperature, pollen prepared with proper technique averaged well above 80% germination within 3-4 hours. The sucrose solution may be kept under refrigeration for future use, although best results were obtained from freshly prepared solutions.