RESEARCH REPORTS TGC REPORT 52, 2002
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A rise of productivity of transgenic tomato (Lycopersicon esculentum Mill.) by
transfer of the gene iaglu from corn
1Rekoslavskaya N. I., 1Salyaev R. K., 2Mapelli S., 1Truchin A. A., 1Gamanetz L. V.
1Siberian Institute of Plant Physiology and Biochemistry, Siberian Branch of RAS, PO
Box 1243, Irkutsk, Russia, e-mail: phytolab@sifibr.irk.ru
2Istituto Biologia Biotecnologia Agraria, C.N.R., via Bassini 15, Milan, Italy, e-mail
mapo@ibv.mi.cnr.it
The creation of transgenic plants with the aim to increase productivity and to gain
resistance to unfavorable natural and abiotic factors as a result of transgenesis is
currently a promising task. The transfer and integration of the maize iaglu gene into
Solanum plants was shown earlier to improve an auxin status expressed in an elevated
content of free and bound indoleacetic acid (IAA) and accelerated growth and root
formation in transgenic plants (Zhukova et al. 1997; Gamanetz et al. 1998).
Tomato seeds, of Ventura and Verlioka varieties, obtained from in vitro transformed
plant were utilized to confirm gene insertion and evaluate the effect on plant morphology
and productivity. Tomato seedlings cv. Bumerang were infected with transconjugant in
planta without step of cultivation in vitro. The efficiency of plant transformation was
assessed by the activity of the markers and target enzymes, ƒÀ-glucuronidase (GUS)
(Table 1), neomycinphosphotransferase (Table 2) and UDPG-transferase, respectively
in leaves of adult plants.
Table 1. The activity of b-glucuronidase in leaves of tomato L. esculentum cv.
Ventura
Fluorescence Impulses
(10 4/mg protein/hr)
Control
6.2
Transgenic
428.6
The data from Sephadex G-25 column eluates of tomato leaves enzyme extracted
in K/Na phosphate buffer.
In crude extracts from leaves of transgenic tomato cv. Verlioka, the activity of GUS
was 284.1±59.1 x 10 4 fluorescence impulses/gram of dry matter, comparing to the
activity of 62.5±4.2 x 10 4 fluorescence impulses/gram of dry matter measured in the
controls.
The transgenesis of tomato hybrid plants Bumerang was confirmed by expression of
GUS-activity with color substrate 5-bromo-4-chloro-3-indolyl- ƒÀ-glucuronide due to the
appearance of blue zones in trichoblasts on stems of transgenic plants after incubation
of small cuttings.
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