RESEARCH REPORTS TGC REPORT 52, 2002
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found that amounts of Lys, Arg, Asp, Glu, Val, Met, Leu, iLeu, Tyr and Phe were higher
in transgenic fruits and the content of Pro, Gly and Ala were lower. If the change in
amino acids composition in fruit and the increase in vitamin C can be accompanied with
change in other substances (i.e. carotenoids) and influence the fruit nutritional value will
be a point to investigate. Larger pool of lower molecular weight compounds, such as
amino acids, in plants has the role to balance the osmotic pressure in cells in order to
overcome water stress.
Table 2. Total amino acids contents in leaves and fruits of tomato cv. Ventura
(nmol/g fresh weight).
Leaves
Green fruits
Red fruits
Control
4206.2
5208.4
5557.5
Transgenic
4931.3
6349.1
8899.5
Leaves of transgenic plants cv Ventura contained more water then the control
(Table 1), perhaps the abundance of low molecular weight compounds balanced the
osmotic pressure in cells in order to maintain water content high and to overcome
drought and water stress.
Measurement of leaf gas exchange (Table 3) indicated that the Ventura transgenic
plants have higher net carbon dioxide assimilation and lower stomatal conductance and
water transpiration indicating a possible higher efficiency of water use.
Table 3. Comparison of leaf gas exchange between control and transgenic tomato.
Net
photosynthesis
Water vapor
transpiration
Stomatal
Conductance
mmol m-2s-1
mmol m-2s-1
mol m-2s-1
30th June
Control
11.20±0.2199
11.07±0.003483
1.791± 0.7129
Transgenic
13.35±0.2610
9.073±0.003649
0.8248±0.1983
31st July
Control
12.56±0.6676
12.40±0.1264
1.647±0.6759
Transgenic
15.55±0.4139
9.888±0.09210
0.8225±0.2395
During Siberian summer the tomato cultivation occurred under a plastic greenhouse
and hot temperatures occurred sometimes. In this condition both control and transgenic
plants wilted but the recovery was faster in transgenic tomato plants than in control
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