RESEARCH REPORTS TGC REPORT 52, 2002
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polymorphic primers. On bulks, primer OPU3 (5’-CTATGCCGAC-3’ with a
molecular weight of 2979 bp) showed polymorphism between bulks. With OPU3
primer, a DNA fragment of 1500 bp (designed OPU3 1500) was amplified and by
agarose gel electrophoresis, a well defined band present in the susceptible bulk
but not in the resistant bulk was observed (Fig. 1). The OPU3 marker was closely
linked with susceptibility to O. lycopersici.
Literature cited
Lindhout P. and Pet G., 1990. Resistance to Oidium lycopersici in Lycopersicon
species. Tomato Genetics Cooperative Report 40, 19.
Michelmore R. W., Paran I., Kesseli R. V., 1991. Identification of markers linked
to disease-resistance genes by bulked segregant analysis: a rapid method to
detect markers in specific genomic regions by using segregating populations.
Proceedings of the National Academy of Sciences USA 88: 9828-9832.
Table 1. - Observed segregation for powdery mildew resistance of LC-95 line of
Lycopersicon esculentum var. cerasiforme and goodness of fit test.
Number of plants
Pedigree
Ra
S
Expectedb
ratio
ƒÔ2
P
LC-95
72
0
72:0
-
-
Super Marmande
0
97
0:97
-
-
F1
0
96
0:96
-
-
F2
30
70
25:75
1.33
0.25-0.30
BC-R
49
51
50:50
0.04
0.80-0.90
BC-S
0
100
0:100
-
-
aR =resistant and S = susceptible
bAssuming a single recessive gene for resistance
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