tissue
(2n
36)
of
the
cross
L
.
hirsutum
f
.
typicum
(2n
48)
X
L
.
peruvianum
(2n
24)
.
The
meiotic
division
was
abnormal
as
expected
with
aneuploids
.
The
stainability
of
the
pollen
was
between
28
to
72
%
.
The
obtained
results
show
that
chromosome
elimination
takes
place
during
in
vitro
plant
regeneration
from
somatic
triploid
tissue
of
the
sesquidiploid
L
.
hirsutum
f
.
typicum
(2n
48)
X
L
.
peruvianum
(2n
24)
.
In
10
(30%)
of
the
produced
regenerants
the
2n
(24)
chromosomes
of
L
.
hirsutum
f
.
typicum
were
eliminated
while
the
n
(12)
chromosomes
of
L
.
peruvianum
were
doubled
and
dihaplid
L
.
peruvianum
plants
were
produced
.
Further
studies
are
in
progress
to
apply
this
approach
for
the
production
of
dihaploids
from
other
species
of
genus
Lycopersicon
.
Literature
cited:
1
.
Murashige
T
.
,
Skoog
.
1962
.
Physiol
.
Plant
.
15,
73
-
79
.
2
.
Rick
C
.
1963
.
Evolution
17,
216
-
232
.
3
.
Rick
C
.
1979
.
In:
Hawkes
J
.
,
Lester
R
.
,
Skelding
A
.
(ed
.
)
The
Biology
and
Taxonomy
of
the
Solanaceae
.
Acad
.
Press
London,
pp
.
667
-
677
.
4
.
Upadhya
M
.
,
Majid
R
.
1964
.
Ind
.
J
.
Genet
.
Pl
.
Breed
.
,
24,
3244
-
251
.
5
.
Vulkova
-
Achkova
Z
.
,
Stoeva
P
.
1978
.
Compt
.
Rend
.
Acad
.
Bulg
.
Sci
.
,
31,1,109
-
111
.