complete
plant
at
the
end
of
the
experiment
were
considered
viable
embryos
.
The
results
are
summarized
in
the
next
table
.
Table
1
.
Viable
embryos
obtained
in
four
crosses
between
L
.
esculentum
x
L
.
peruvianum
CROSS
VIABLE
OVULES
(%
AVERAGE)
TOTAL
PLANTLETS
(AVERAGE)
Moneymaker
X
LA
98
0a
0
Muchamiel
X
LA
98
15,99911054b
28
Moneymaker
X
LA
1278
11,42857143b
9
Muchamiel
X
La
1278
26,19838617b
23
Means
followed
by
a
different
letter
are
different
with
p>
0
.
95
.
Moneymaker
as
the
maternal
parent
gave
significantly
less
viable
embryos
than
Muchamiel
.
The
influence
of
the
maternal
genotype
on
the
percentage
of
viable
embryos
has
also
been
noted
by
Sacks
et
al
.
(1998),
however,
the
number
of
plants
recovered
in
their
experiment
was
much
lower
than
in
our
case,
perhaps
because
they
did
their
pollination
with
a
bulked
mixture
of
four
L
.
peruvianum
accessions
.
A
viability
so
high
as
in
our
case
(25%
of
viability
and
5
-
28
plants
obtained),
has
only
been
registered
before
in
the
work
of
Lan
Zhuang
&
Adachi
(1996),
but
they
used
a
different
culture
medium
and
their
rescue
technique
was
more
complex
than
ours
.
The
increase
of
the
embryo
viability
obtained
in
our
case
could
be
attributed
to
the
conjunction
of
the
following
factors:
a)
Abundant
pollination
in
each
cross
.
b)
Moderately
aggressive
disinfectant
procedure
in
comparison
with
procedures
described
by
(Imanishi
(1991),
Chen
&
Adachi
(1996),
Imanishi
et
al
.
(1996),
where
at
least
two
desinfection
steps
with
bleach
were
used
.
c)
Induction
medium
(OMS
medium)
with
a
higher
concentration
of
vitamins
than
other
commonly
used
media
(Imanishi,
1991;
Chen
&
Adachi,
1996;
Imanishi
et
al
.
,
1996;
Doganlar
et
al
.
,
1997;
Sacks
et
al
.
,
1998)
.
Acknowledgements
:
This
work
has
been
founded
by
PIR
-
28
(Dir
.
Gral
.
Investigacion
Agraria,
Consejeria
de
Agricultura,
Junta
de
Andalucia)
.
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