Sources
of
high
soluble
solid
and
vitamin
C
content
from
Lycopersicon
pimpinellifolium
are
interesting
in
breeding
for
internal
quality
of
fresh
market
tomato
Roselló,
S
.
,
Galiana
-
Balaguer,
L
.
and
Nuez,
F
.
Biotechnology
Dept
.
Universidad
Politécnica
de
Valencia
(UPV)
.
Cno
.
Vera,
14;
46022
Valencia
.
Spain
Currently,
improvement
for
internal
quality
(nutritive
and
organoleptic)
is
one
of
the
main
breeding
objectives
for
fresh
market
tomato
.
Flavour
and
nutritive
value
are
complex
characteristics
because
they
are
conditioned
by
the
content
of
many
chemical
compounds
of
tomato
fruits
.
The
precise
analytical
determination
of
each
of
these
compounds
could
become
a
very
laborious
task
.
However,
a
preliminary
screening
trial
can
be
performed
to
determine,
by
quick
analyses,
the
main
groups
of
compounds
responsible
for
internal
quality
in
tomato
.
It
would
permit
the
preselection
interesting
accessions
and
it
would
greatly
reduce
the
number
of
detailed
subsequent
characterizations
with
more
precise
techniques
.
Screening
L
.
pimpinellifolium
germplasm
for
soluble
solids
content
(SSC),
titratable
acidity
and
vitamin
C
content
is
the
objective
of
this
work
.
Twenty
accessions
of
L
.
pimpinellifolium
collected
in
Ecuador
and
Peru
by
the
Vegetable
Genetics
Breeding
Group
(Politechnic
University
of
Valencia)
were
tested
(Table
1)
.
Three
experimental
breeding
lines
of
tomato
(FLA7060,
NEMA
-
R
and
NE
-
1),
two
Spanish
varieties
of
processing
tomato
(GEVORA
and
GUADAJIRA)
and
one
commercial
hybrid
(CAMBRIA,
Seminis
Vegetable
Seeds,
Almería,
Spain)
were
used
as
controls
.
Plants
were
cultivated
in
the
greenhouse
during
spring
.
Fruits
were
collected
at
the
completely
mature
stage
.
SSC
was
determined
with
a
refractometer
(ATAGO
-
N1
Tokyo,
Japan)
and
the
results
are
reported
as
ºBrix
at
20
ºC
.
Vitamin
C
content
and
titratable
acidity
were
determined
immediately
after
blending
and
made
from
aliquots
of
the
homogenized
sample
.
Vitamin
C
content
was
titrated
with
2,6
dichloroindo
-
phenol
.
Total
acidity
was
titrated
with
0
.
1
N
NaOH
and
phenoftalein
(AOAC,
1990)
.
Screened
accessions
have
shown
a
wide
range
of
variation
for
SSC
and
vitamin
C
(Table
1)
.
The
range
of
variation
for
titratable
acidity
was
smaller
.
The
most
interesting
accessions
were
UPV
-
17039,
UPV
-
16978,
UPV
-
16984,
UPV
-
16982
and
UPV
-
16985
because
their
SSC
was
twice
the
value
of
the
controls
and
sometimes
more
.
The
ratio
SSC/Titratable
acidity
seems
to
suggest
that
the
taste
of
UPV
-
17039,
UPV
-
16984
and
UPV
-
16985
is
slightly
sweeter
than
that
of
the
controls,
while
UPV
-
16978
and
UPV
-
16982
have
approximately
the
same
taste
as
the