Resistance
to
Oidium
lycopersicum
:
allelism
test
between
Lycopersicon
hirsutum
G1
.
1560
and
L
.
hirsutum
PI247087
Moretti,
A
.
and
Caranta,
C
.
INRA,
Genetics
and
Breeding
of
Fruits
and
Vegetable,
Dom
.
St
Maurice,
BP94,
84143
Montfavet
cedex,
France
.
E
-
mail
:
Carole
.
Caranta@avignon
.
inra
.
fr
Resistance
to
powdery
mildew
(
Oidium
lycopersicum
)
in
Lycopersicon
hirsutum
G1
.
1560
was
shown
to
be
controlled
by
an
incompletely
-
dominant
gene,
Ol
-
1
,
mapped
on
chromosome
6
in
the
vicinity
of
the
resistance
genes
Mi
/
Meu
-
1
,
Cf
-
2
/
Cf
-
5
(van
der
Beek
et
al
.
1994)
.
Among
the
accessions
of
wild
species
frequently
used
at
INRA,
a
very
high
level
of
resistance
to
O
.
lycopersicum
was
identified
in
L
.
hirsutum
PI247087
.
The
intermediate
levels
of
resistance
observed
during
the
introgression
of
PI247087
resistance
into
elite
tomato
lines
attested
to
the
oligo
-
or
polygenic
nature
of
this
resistance
.
Moreover,
fruits
from
all
the
plants
with
the
higher
level
of
resistance
were
yellow
.
An
allelism
test
between
these
plants
and
a
tomato
line
homozygous
for
the
r
gene
(yellow
flesh)
was
performed
and
revealed
a
linkage
between
r
and
resistance
to
O
.
lycopersicum
(Laterrot
&
Moretti,
unpublished
data)
.
To
determine
whether
a
part
of
PI247087
resistance
to
O
.
lycopersicum
is
due
to
the
gene
Ol
-
1
,
an
allelism
test
was
performed
between
PI247087
and
L
.
hirsutum
G1
.
1560
.
Resistance
of
L
.
esculentum
Momor,
L
.
hirsutum
PI247087
and
G1
.
1560,
the
F1
hybrids
(Momor
X
PI247087)
and
(PI247087
X
G1
.
1560)
and
the
F2
progeny
(PI247087
X
G1
.
1560)
to
O
.
lycopersicum
was
evaluated
during
two
independent
tests
.
Plants
were
inoculated
three
weeks
after
sowing
by
spraying
with
a
suspension
of
10
4
conidia
ml
-
1
.
Inoculum
was
prepared
from
freshly
sporulating
leaves
of
heavily
diseased
tomato
plants
.
After
inoculation,
plantlets
were
transferred
into
growth
chamber
with
100%
relative
humidity,
24°C
day/18°Cnight
and
12
hours
light
.
Disease
was
evaluated
18
days
after
inoculation
according
to
the
following
index
:
0=no
visible
sporulation
(healthy
plants),
1=very
few
powdery
mildew
spots
surrounded
by
a
necrosis
(stopped
sporulation),
2=moderate
number
of
spots
(moderate
sporulation),
3=very
high
number
of
spots
(strong
sporulation)
.
Plants
classified
0
or
1
were
considered
resistant
and
plants
classified
2
or
3,
susceptible
.
Results
are
presented
in
Table
1
.
All
plants
could
be
classified
unambiguously
.
L
.
esculentum
Momor
was
completely
susceptible
with
most
of
the
plants
with
a
high
number
of
sporulation
spots
.
L
.
hirsutum
PI247087
and
G1
.
1560
were
resistant
with
most
of
the
plants
without
sporulation
.
A
small
number
(3/15)
of
the
F1
(Momor
X
PI247087)
showed
some
sporulation
.
The
same
observation
was
made
by
van
der
Beek
et
al
.
(1994)
on
the
F1
hybrid
between
G1
.
1560
and
a
susceptible
L
.
esculentum
line
.
On
the
contrary,
all