A comparison of the effects of thermal neutrons and X-rays on germination of irradiated tomato seeds.

Mertens, T. R.

Lines 018 and 003 of L. esculentum and 206 and 226 of L. pimpinellifolium provided by A. D. Burdick were irradiated at Brookhaven National Laboratory and germination studies were then made. In the table below percent survival is expressed in terms of the number of seeds planted.

   
  Effect of thermal  neutrons  and X-rays on  germination  of  
                irradiated  tomato-seeds
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Line               Hours   N\th\*         
       0    10    20    30    40    50    
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                Percent Germination
206   99    95    89    90    93    67    
226  100    97    97    23     0     0    
018   94    98    98    96    90    62    
003   90    83    83    77    21     2    

                 Percent Survival
206    -     -     -     -     -     -    
226  100    93    93    23     0     0    
018   94    98    98    96    82    40    
003   90    83    83    54     4     0    

             Mean Days to Germinate
206  6.0   6.0   6.7   8.8   9.0   10.3   
226  6.5   6.6   7.0   8.3     -      -   
018  6.0   6.2   6.6   6.9   8.5   9.9  
C03    -     -     -     -     -     -  
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Line             1000r X-ray
       6    12    18   24   36   48   60
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             Percent Germination
206    -     -     -    -    -    -    -
226  100   100   100   87   47   13    3
018   96    98   100   96   94   98   96
003    -     -     -    -    -    -    -   

              Percent Survival
206    -     -     -    -    -    -    -
226  100   100   100   87   43   10    0
018   96    98   100   96   94   98   96
003    -     -     -    -    -    -    -    

           Mean Days to Germinate
206    -     -     -    -    -    -    -
226    6.4   6.2   6.5  7.3  8.5 10.0  8.0
018    5.7   5.7   5.7  6.0  6.4  6.1  6.5
003    -     -     -    -    -    -    -
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 * at flux of Ca  8xlO^8  N\th\ /sq. cm./sec.

The table shows different responses of lines within and between species when the dormant seeds were irradiated as specified. Line 018 was especially remarkable in its ability to survive high doses of both types of irradiation. Furthermore, fruit was set and seed obtained from all treatments of 018 when the irradiated plants were crossed to the unirradiated controls. The irradiated plants were used successfully as both pollen and seed parents in these crosses. It is clear that increasing doses of thermal neutrons tend to delay germination, but delayed germination of X-irradiated seed occurs only when higher doses are used.