accumulating
plants
to
select
for
the
best
plants
to
use
in
a
breeding
program
.
The
bias
would
also
be
important
if
the
type
of
acylsugar
underestimated
by
the
Rhodamine
assay
were
particularly
important
for
acylsugar
efficiency
or
effectiveness
.
False
negatives
would
be
important
if
it
is
necessary
to
detect
every
acylsugar
producing
plant
in
a
population
.
Despite
the
bias
in
the
Rhodamine
B
assay
there
could
be
instances
in
which
the
assay
would
be
of
value
.
An
example
is
its
use
in
our
1998
winter
greenhouse
survey
.
Since
the
Rhodamine
B
assay
can
be
performed
on
younger
plants,
we
grew
a
very
large
initial
populations
(filling
the
greenhouse
even
as
small
plants),
used
the
Rhodamine
assay
in
a
preliminary
screen
and
reduced
the
populations
size
to
that
the
greenhouse
could
contain
as
mature
plants
by
saving
only
the
positive
plants
.
The
plants
saved
were
then
screened
by
the
Nelson’s
copper
reagent
assay
at
15
weeks
to
determine
relative
acylsugar
levels
.
We,
no
doubt,
eliminated
acylsugar
-
producing
plants
in
the
first
screen
due
to
false
negatives
common
in
the
Rhodamine
test,
however
all
of
the
plants
which
indicated
as
being
positive
by
the
Rhodamine
-
based
test
were
also
positive
by
the
Nelson
-
based
assay
.
This
approach
allowed
us
to
maximize
the
number
of
acylsugar
-
accumulating
plants
recovered
in
that
generation
in
a
restricted
area
of
greenhouse
space
.
The
use
of
the
Rhodamine
test
did
not
eliminate
the
need
to
use
the
Nelson
-
based
assay
.
Literature
Cited:
Goffreda,
J
.
C
.
,
Steffens,
J
.
C
.
,
Mutschler,
M
.
A
.
1990
.
Association
of
epicuticular
sugars
with
aphid
resistance
in
hybrids
with
wild
tomato
.
J
.
Amer
.
Soc
.
Hort
.
Sci
.
115:161
-
165
.
Lin,
Y
.
,
Wagner,
G
.
J
.
1994
.
Rapid
and
simple
method
for
estimation
of
sugar
esters
.
Journal
of
Agricultural
and
Food
Chemistry
VOL:1709
-
1712
.
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