Putative developmental mutants isolated from EMS and fast neutron mutagenized seed pools Ustach, C . V . , Hu, G . , and Baker, B . J . Department of Plant Biology and Microbiology, University of Berkeley, and Plant Gene Expression Center, USDA, 800 Buchanan Street, Albany, CA 94710 Our lab is primarily concerned with disease resistance; however, during our screen for disease resistance mutants, we isolated three putative developmental mutants from our EMS mutagenized and fast neutron mutagenized seed populations .   We present these plants here with brief descriptions, so that any lab who is interested may obtain these lines from us for further study .   All mutants are from a VF36 background, and are transgenic for the N TMV disease resistance gene . Line Mutagen Description Conferred to next Generation? NST10 EMS Putative leaf mutant . Variegated green/yellow patterns on leaves resembling mosaic - disease symptoms, even though plant is not infected with virus . Is not impaired in TMV resistance . Sets fruit, but has reduced fertility . Yes NST43 EMS Dwarfed/small in stature . May have reduced number of trichomes . Appears to have increased rate of senescence in leaves . Fruit set does not appear to be affected . Yes FN3/1 Fast Neutron "Cabbage/kale" mutant: Leaves are packed close together along the main stem of the plant and all stems of leaves/leaflets are very thick . Leaflets are tightly curled under . Plant does set fruit, but seed has not been collected yet . Not determined

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