Primer
set
F
sequence
R
sequence
T20P8
.
A
AGATCAGCTCACCGAAGATCA
TGGCCATCATGACCTTAACA
T15B16
.
A
GGTGTTGGGAGATCCTGATG
CAACTGCCCAAATCCCTTTA
F4L23
.
19
TGGAGCGATTTGGTGTCTTTG
TGCAGTAGTCTGACCCTTCAACAAC
T13M11
.
A
GCAACAGTGAGCATGTCAAAA
CCAAGATTGCAATAGCAGCA
MJB24
.
A
ATCCAATCCAGCACAGGAAG
AAAGCAGGAGCATCGTTCAC
F4L23
.
20
ACCTGGTTTCCTTGGTGGTAGTG
CTTTAGTTCCTCAGCAGCCTTGAC
Table
1
.
Primer
sequences,
5'
-
3'
Enzyme
cleavage
site
CAPs
detected
%
success
Tru9I
TTAA
0/8
0%
Tsp509I
AATT
1/8
13%
RsaI
GTAC
2/9
22%
EcoRV
GATATC
1/9
11%
DraI
TTTAAA
1/9
11%
HaeIII
GGCC
0/9
0%
AluI
AGCT
0/9
0%
TaqI
TCGA
2/8
25%
MspI
CCGG
1/8
13%
HinfI
GANTC
1/8
13%
Table
2
.
Enzymes
used
and
their
success
rate
in
identifying
polymorphisms
.
Literature
cited:
Konieczny
A,
Ausubel
FM
(1993)
A
procedure
for
mapping
Arabidopsis
mutations
using
co
-
dominant
ecotype
-
specific
PCR
-
based
markers
.
Plant
J
4:
403
-
410
No navigation control above? Click here!
