encodes
a
protein
targeted
to
the
chloroplast
.
In
an
F
2
L
.
esculentum
cv
.
VF36
x
L
.
pennellii
LA716
population
(F2
-
LP),
the
LeME2
probe
segregated
12
+/+
:
35
+/P
:
22
P/P,
consistent
with
the
expected
1:2:1
ratio
(
X
2
=
2
.
9)
.
LeME2
mapped
to
approximately
the
same
region
of
chromosome
5,
where
it
showed
tight
linkage
to
TG379
(Fig
.
1)
.
These
results
indicate
the
LeME2
cDNA
and
Mae
-
1
isozyme
locus
very
likely
correspond
to
the
same
gene
.
Although
we
haven’t
surveyed
other
Lycopersicon
species
for
Mae
-
1
polymorphism,
we
anticipate
that
in
certain
wide
crosses
this
gene
could
provide
a
useful
new
isozyme
marker
for
the
long
arm
of
chromosome
5
.
Figure
1
.
Genetic
maps
of
chromosome
5,
from
BC
1
L
.
esculentum
x
S
.
lycopersicoides
(BC
-
LS)
and
F
2
L
.
esculentum
x
L
.
pennellii
(F2
-
LP)
populations,
showing
the
location
of
a
malic
enzyme
gene
determined
through
segregation
of
the
isozyme
(
Mae
-
1
)
or
cDNA
(LeME2)
.
Literature
cited:
Chetelat,
R
.
T
.
,
P
.
Cisneros,
L
.
Stamova,
C
.
M
.
Rick
.
1997
.
A
male
-
fertile
Lycopersicon
esculentum
x
Solanum
lycopersicoides
hybrid
enables
direct
backcrossing
to
tomato
at
the
diploid
level
.
Euphytica
95:
99
-
108
.
Weeden,
N
.
F
.
,
J
.
F
.
Wendel
.
1989
.
Genetics
of
plant
isozymes
.
In:
D
.
E
.
Soltis
&
P
.
S
.
Soltis
(eds)
Isozymes
in
Plant
Biology
.
Dioscorides
Press,
Portland
.
pp
.
46
-
72
.
Wendel,
J
.
F
.
,
N
.
F
.
Weeden
.
1989
.
Visualization
and
interpretation
of
plant
isozymes
.
In:
D
.
E
.
Soltis
&
P
.
S
.
Soltis
(eds)
Isozymes
in
Plant
Biology
.
Dioscorides
Press,
Portland
.
pp
.
5
-
45
.
TG623
9
.
9
TG432
23
.
2
TG379
7
.
6
Mae
-
1
10
.
2
TG23
11
.
7
TG238
CD64
9
.
5
CT93
15
.
8
TG318
5
.
4
TG379,
LeME2
8
.
7
TG358
14
.
1
TG23
19
.
1
TG69
BC
-
LS
F2
-
LP
Chromosome
5
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