To
determine
empirically
the
genome
coverage
of
the
BAC
library,
we
screened
it
with
5
single
copy
RFLP
markers
on
chromosome
12
(kindly
provided
by
S
.
Tanksley,
Cornell
University)
.
Table
1
shows
the
result
of
the
screening
from
the
first
three
filters
.
In
every
case,
at
least
5
clones
were
identified
.
Table
1
.
The
average
number
of
hits/probe
for
each
filter
(2
.
14
genome
coverage/filter)
RFLP
marker
No
.
of
filters
screened
Total
No
.
of
hits
Average
No
.
of
hits/filter
Expected
No
.
of
hits/filter
CD4
3
9
3
2
.
14
CD22
3
9
3
2
.
14
TG387
3
11
3
.
7
2
.
14
TG394
3
5
1
.
7
2
.
14
TG618
3
12
4
2
.
14
Total
15
46
3
.
06
2
.
14
BAC
library
access
and
distribution
The
tomato
BAC
library
was
constructed
with
funds
provided
by
the
USDA
NRI
program
and
therefore
is
freely
available
to
the
research
community
.
The
library
is
made
available
through
our
BAC
Resource
Center
at
CUGI,
either
as
a
set
of
high
density
hybridization
filters
or
individual
clones
(http://www
.
genome
.
clemson
.
edu)
.
The
BAC
Resource
Center
has
received
NSF
funding
for
the
next
five
years
to
provide
services
to
the
genomics
community
.
The
entire
library
is
gridded
onto
seven
500
cm
2
Hybond
N+
filters
(Amersham,
USA)
.
Each
filter
contains
18,432
independent
BAC
clones
plated
in
duplicate
.
The
duplication
pattern
aids
in
clone
identification
and
provides
a
positive
control
for
each
positive
hybridization
signal
.
Figure
2
shows
an
example
of
such
a
filter
hybridized
with
the
tomato
marker
CD22
.
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