pi-2 -- a novel pistillate mutant

Rick, C.M. Dept. of Veg. Crops, Univ. of California, Davis, CA 95616

Pistillate-2 (pi-2) originated from EMS treatment of cv. Castlemart. Carrying a stock number of 3-802, it was given a provisional name of "green pistillate", which some investigators who utilized it might recognize. The M1 and subsequently produced F1's had normal phenotype. The recessive homozygotes have a unique phenotype. Plant size and vigor are reduced. A tendency toward inflorescence abortion often delays flowering, and casual examination might fail to detect the reduced, inconspicuous inflorescence. Instead of normal flowers, small structures appear that have only calyx and pistil. At anthesis the calyx appears juvenile, failing to elongate and spread its segments but remaining tightly adpressed to the pistil. Thus, in these morphological respects, pi-2 differs from pi, whose flowers have a corolla, albeit highly modified, and open sufficiently to permit exposure of the stigma. Application of viable pollen results in irregular fruit set and subnormal seed production. Consequently pi-2 is not the answer to the breeder's prayer for a functional male sterile mutant useful for hybrid seed production.

The original M2 segregated 15 + : 2 pi-2. Additional F2's and BC's were generated from F1's of pi-2/pi-2 x normal Castlemart. A series of 7 F2's yielded a total of 82 + and 20 mutant phenotypes, a deficiency of the latter being consistent in most families. For small BC families were obtained with totals of 25 + and 12 pi-2/pi-2. The deficiency of mutant homozygotes, characteristic of both generations, is not unusual for induced mutations, especially those of weaker constitution. It therefore seems reasonable to assume that a single recessive gene is responsible for this trait. Limited seed supplies are available for distribution to interested parties.