Sterility caused by loss of the nuclear wall in early prophase of L. peruvianum.

Lesley, M. M.

A very vigorous plant from an open-pollinated line of L. peruvianum PI 126, 946 (48.074.1) has pale yellow flowers which usually fail to open fully. No normal pollen is produced. In pre-leptotene or zygotene the nuclear wall in the PMC breaks and masses of Feulgen-positive material appear suddenly in its place, together with a few fibers. The number of these masses varies from one to many; usually there are from 3-7 which vary greatly in size and shape. A more or less normal spindle may be formed and cell division occurs, giving a majority of dyads but also triads, tetrads, and polyads. In late October of 1950 occasional cells were found to have 24 unpaired chromosomes. Embryo sac mother cells taken at the same time had 24 single chromosomes scattered upon the spindle. More flowers tend to open fully late in the growing season. Sibs of 48.074.1 had flowers which opened normally. Meiosis was normal and the plants were very fruitful.

The behavior of D.N.A. in 48-074.1 indicates that it is in a semifluid condition in early prophase.