Mutant
scions
grafted
onto
normal
rootstocks
do
not
suggest
that
the
precursors
of
the
anthocyanin
pigment
are
translocated
in
the
xylem,
and
reciprocal
grafts
have
not
revealed
inhibitor
action
of
a
translocatable
nature
.
Progeny
derived
from
fruits
borne
on
the
grafted
anthocyaninless
sh
.
iots
lacked
anthocyanin
.
Addition
of
varying
concentrations
of
trans
-
cinnamate
to
germinating
seeds
(by
soaking
the
blotter),
to
shoot
tissues
(by
spray
treatments),
and
to
cuttings
(via
the
rooting
solution)
have
thus
far
not
resulted
in
pigment
synthesis
.
The
two
ah
mutants
used
in
this
experiment
(C545
and
E128)
were
discovered
by
Farrell
and
Rick
in
a
1979
field
EMS
survey
of
cv
.
VFNTCh,
and
allelism
with
the
chromosome
9
ah
marker
(chr
9L
-
24)
has
now
been
confirmed
.
Transcriptional
differences
induced
by
the
EMS
modification
of
the
DNA
template
could
lead
to
a
configuration
change
in
a
protein
such
as
the
PAL
enzyme
.
Biochemical
investigations
indicate
that
the
PAL
enzyme
in
potato
cultivars
is
a
tetramer
.
A
failure
of
the
altered
pacemaker
enzyme
to
effectively
recognize
and
bind
the
substrate
could
be
reflected
in
a
reduction
in
enzymic
efficiency
and
could
result
in
the
kinetically
favored
channeling
of
the
trans
-
cinnamate
product
to
the
coumarin
(ortho
hydroxylase),
lignification
(reductase),
or
conjugate
(transferase)
phenylpropanoid
pathways
at
the
expense
of
the
flavonoid
(synthase)
pathway
which
mediates
anthocyanin
synthesis
.
Holle,
Miguel
and
C
.
M
.
Rick
The
"hygienic"
In
August
-
October,
1980
we
had
the
method
of
collecting
revisited
.
opportunity
to
reevaluate
the
use
of
TP
for
collecting
and
storing
fruits
and
other
plant
parts
while
we
were
collecting
tomato
species
in
Ecuador
and
Peru
.
In
general
our
observations
supported
the
contentions
stated
by
us
in
TGC
29:24
-
25,
particularly
those
relating
to
the
Holle,
Rick,
and
Fobes'
Inverse
Quality
Rule
(utility
for
plant
collecting
purposes
is
inversely
related
to
customer
appeal)
.
We
were
very
pleased
to
report
that
the
TP
supply
situation
in
both
countries
has
not
deteriorated;
brands
ideal
for
our
purposes
were
still
available
and
in
good
supply
.
Our
travels
afforded
an
unusually
good
opportunity
to
test
a
large
assortment
of
brands;
furthermore,
besides
rolls
that
we
purchased
in
tiendas
and
supermercados,
additional
categories
for
evaluation
were
on
display
in
the
bathrooms
of
our
lodging
places
.
We
nevertheless
regret
to
report
that
certain
brands
are
not
being
produced
with
satisfactory
quality
control
.
We
discovered,
for
example,
that
the
paper
might
not
be
continuous,
but
