15 RESEARCH NOTES TGC REPORT No . 31, 1981 We tried to check different testing methods in order to study the influence of low night temperature on pollen maturity using 42 F2 hybrids between resistant and susceptible varieties . We have used as a source of low temperature adaptation Sub Arctic Delighted crossed with greenhouse and field varieties . The hybrids ware grown in a plastic greenhouse in the spring and fall . The night temperature during the opening up of the first 3 inflorescences was 5 - 10oC . We compared the rate of plant growth with the number of points given for the blossom and fruit set ability (N . Musat method) . We have also analysed the pollen viability with acetocamine and germination in vitro (sucrose 15%) . We have also tried to compare our results with seed germination in high osmotic pressure solution - KNO3, 1% . Some seeds of two lines germinated in 60 hours but most lines and hybrids were inhibited and germinated after 5 - 8 days . We have found a significant relation between the rate of growth and the number of points accorded for blossom and good fruit - set ability for most of the hybrids . The acetocarmine test was not significant, but the germination of the pollen in vitro was much more conclusive and related with the fruit setting at low tempera - tures . The germination of seeds in 1% solution of KNO3 can be used as an efficient method of selection for cold set ability or generally for non - favorable conditions, though it is not strictly related with low temperature adaptation . It seems to us that cold resistance is not absolutely related with low temperature fruit setting and may be under control of different groups of genes . Rao, R . N . and Panuganti N . Rao Pachytene Details on the frequency and chromosome pairing in a sesqui - distribution of partner exchanges for diploid Lycopersicon esculentum (4n) the various chromosome sets in the x L . peruvianum var . glandulosum triploid hybrid L . esculentum cv . (2n) . Marglobe (4n) x L . peruvianum var . glandulosum (2n) were studied in comparison with the observations of a similar nature in the autotetraploid L . esculentum var . Marglobe to a