Linkage tests with the mutant clausa (clau).
Butler, L.
From the seeds supplied by H. Stubbe, plants have been grown and checked for phenotype and then crossed with various tester stocks. The first results are given below (all crosses are in the repulsion phase):
a b c d Chi square linkage clau X d\1 609 257 194 30 22.9 clau X r 210 87 77 18 4.08 clau X c 784 272 206 23 11.59 clau X a 809 225 165 59 2.55 clau X rv 454 149 141 17 10.1It is obvious that clausa cannot be in all three groups indicated and that the individual ratios have to be checked for irregularities. In the first cross there is a shortage of some 38 clau d\1 segregates. If these can be attributed to misclassification or reduce viability, then there is no linkage. However, since the excess of + d\1 segregates suggests linkage, further tests are necessary. The segregation for linkage with c shows a great lack of clau segregates in both classes. The clau c plants are hard to recognize, and if these were wrongly placed in the + c ilass, this would account for both the disturbed ratio and the linkage. The linkage with rv is again the result of the shortage of the double recessive class, and since there is no corresponding increase in either of the middle classes, this is probably not true linkage. I also have fragmentary data which indicate no linkage with y, l\1, or ah, so clau is probably not on chromosomes 1, 2, 3, 6, 8, 9, or the one with rv.