Continued linkage tests with mutants of Stubbe's group I.

Rick, C. M. and F. W. Martin

The program first reported in TGC 10 has been continued, following the same procedures. Seven new linkages have turned up, and two reported last year have been thrown in the doubtful column. A summary of tests completed between the seven and an assortment of various markers is given in the following table. Linkages are indicated by L. suggested but non-significant indications of linkage by S, and no significant departures from random recombination by X. Data for the last two categories are not presented in order to conserve space, but will be provided for anyone who might want them. The suggested linkage between inv and pr gave inconsistent values in different families primarily because the phenotypic interaction between them is difficult to work out. Our first test of sit-e gave a strong indication of linkage, but this was not verified in subsequent tests; we can't offer any apparent reason for this inconsistency.

Tester  deb  fla  ful  inv  rust  sit  ven
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a\1      X    X    X    X    X     X    X
ah       X    X    X    X    X     X    X
au                 X    L    X     L
c        X    X    X    X    X     X
d\1      X    X    X    X    X     X
dl       X    X    X    X    X     X    X
e             X    L    X    X     S    L
ff                           X     X
H        X    X    X    X    X     X
l\1                X    X    X     X
La       L    X    X    X    X     X    X
pr            L    S    S          X    X
r                  X    X    X     X
rv            X    X         X          X
sf            X              X
tf       X    X    X    X    X     X    
W\4           X         X    X     X        
y                  X    X    X     X    
yv
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Data for the significant linkage tests and crossover values estimated by the product moment method are given in the following table. Also included are new segregations for combinations (lut-a\1 and sub-a\1 ), which earlier tests suggested were linked. The last tests are considered more trustworthy, partly because we are more familiar with the materials and partly because the new tests were made in the absence of distrubing segregations. The paling tendency of a\1 tends to confuse classification of lut. All segregations were in F2 repulsion.

                                    Adjusted
Combination                         contingency       Mean
  m    t    + +   + t   m +   m t   Chi-square   Co.  co.
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 deb - La     2    61    25     4      62.1            4.0
             13   155    67     4     164.3
 fla - pr   213    76   107    15      13.47     37.0 38.0
            186    61    56     8       3.75     38.5
 ful - e    127    53    57     9       5.60     37.0 31.0
            175    63    74     6      11.59     30.0
             76    33    32     3       5.55     19.5
            318   118   142    23      10.76     38.5
 inv - au   303    98   104    12       9.84     36.0 36.0
 lut - a\1   98    43    38    12       0.48
            241    92    84    34       0.016
            216    88    57    15       1.54
rust - dl    85    39    37     4       5.82     30.5 31.0
             76    46    33     4       8.32     28.5
            117    33    46     4       3.99     34.0
 sit - au   250   116    83     1?     31.47     11.5- 11.5-
 sub - a    118    57    63    27       0.083
             96    36    28     9       0.022
 ven - e    305   148   102     9      25.57     27.5  28.0
            239   111   107    10      23.32     28.5
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Seeds of the mutants and other relevant lines have been sent to the cooperators for tho respective chromosomes.