Continued linkage tests with mutants of Stubbe's group I.
Rick, C. M. and F. W. Martin
The program first reported in TGC 10 has been continued, following the same procedures. Seven new linkages have turned up, and two reported last year have been thrown in the doubtful column. A summary of tests completed between the seven and an assortment of various markers is given in the following table. Linkages are indicated by L. suggested but non-significant indications of linkage by S, and no significant departures from random recombination by X. Data for the last two categories are not presented in order to conserve space, but will be provided for anyone who might want them. The suggested linkage between inv and pr gave inconsistent values in different families primarily because the phenotypic interaction between them is difficult to work out. Our first test of sit-e gave a strong indication of linkage, but this was not verified in subsequent tests; we can't offer any apparent reason for this inconsistency.
Tester deb fla ful inv rust sit ven __________________________________________ a\1 X X X X X X X ah X X X X X X X au X L X L c X X X X X X d\1 X X X X X X dl X X X X X X X e X L X X S L ff X X H X X X X X X l\1 X X X X La L X X X X X X pr L S S X X r X X X X rv X X X X sf X X tf X X X X X X W\4 X X X X y X X X X yv _________________________________________Data for the significant linkage tests and crossover values estimated by the product moment method are given in the following table. Also included are new segregations for combinations (lut-a\1 and sub-a\1 ), which earlier tests suggested were linked. The last tests are considered more trustworthy, partly because we are more familiar with the materials and partly because the new tests were made in the absence of distrubing segregations. The paling tendency of a\1 tends to confuse classification of lut. All segregations were in F2 repulsion.
Adjusted Combination contingency Mean m t + + + t m + m t Chi-square Co. co. ____________________________________________________________ deb - La 2 61 25 4 62.1 4.0 13 155 67 4 164.3 fla - pr 213 76 107 15 13.47 37.0 38.0 186 61 56 8 3.75 38.5 ful - e 127 53 57 9 5.60 37.0 31.0 175 63 74 6 11.59 30.0 76 33 32 3 5.55 19.5 318 118 142 23 10.76 38.5 inv - au 303 98 104 12 9.84 36.0 36.0 lut - a\1 98 43 38 12 0.48 241 92 84 34 0.016 216 88 57 15 1.54 rust - dl 85 39 37 4 5.82 30.5 31.0 76 46 33 4 8.32 28.5 117 33 46 4 3.99 34.0 sit - au 250 116 83 1? 31.47 11.5- 11.5- sub - a 118 57 63 27 0.083 96 36 28 9 0.022 ven - e 305 148 102 9 25.57 27.5 28.0 239 111 107 10 23.32 28.5 ____________________________________________________________Seeds of the mutants and other relevant lines have been sent to the cooperators for tho respective chromosomes.