Linkage tests with mutants of Stubbe's group I.<p>

Rick, C. M. and F. W. Martin.<p>

A systematic attempt is being made to ascertain the respective chromosome for
each of the mutants in Stubbe's first group (Kulturpfl. 5:190-220) that can
readily be identified in the seedling stage.  Approximately 30 have proved to
be workable as seedling mutants in our experience.  Of these, six proved to be
allelic to previously known genes according to determinations by Dr. Stubbe
and ourselves (rob^imm and rob^crisp allelic with d\1, fru with bu, rub with
l\1, tri with tf, and brac with au).  Of the remainder significant indications
of linkage have been detected for nine.  A resume of the tests completed
between these nine and an assortment of various chromosome markers is given in
the following table.  Therein linkages are designated by L. no significant
departures from random recombination, by x.<p>

<pre>
Tester  dil  lut  na  neg  proc  ra  sub  suf  va^virg 

a\1      X    L    X    L    L    X    L    X    X
ah       X    X    X    X    X    X    X    X    X 
c        X    X    X    X    X    X    X    X    X
d\1      L    X    X    X    X    X    X    L    X
d\1      X    X    X    X    X    X    X    X    L
l             X              X    L         X    
H        X         X         X    X    X    X    X
l\1                                              L
La       X    X    L    X    X    X    X    X    X
rv                           X         X        
sf                           X         X    X   
tf       X         X         X    X    X    X    
yv                 X    X    X    X    X         X

</pre>
Data for the significant linkage teste and crossover values estimated by the
product moment method are given in the following table. Data of the other
tests listed in the preceding table are not presented here but can be
furnished to anyone who might be interested.<p>

<pre>
                                        Adjusted 
Combination                             contingency         Mean
  m     t          + +  + t  m +   m t  Chi-square   Co.    Co.

  dil-d\1   F\2-R  152   56   52     1    14.07      15.5
               "   143   72   96     0    39.97       0     11.0
               "   337  158  167     5    37.56      17.0
  lut-a\1      "   133   53   45     6     5.13      35.5
                   176   60   60     7     5.95      35.0   36.5
               "   206   77   79    22     0.88      38.5
   na-La       "     2   55    8    10    16.41
               "    37   99   34    17    22.87      27.8
  neg-a\1      "   172   59   54     0    15.18       0
               "   254  117   83     8    18.02      28.5   26.0
               "   164   66   60     3    14.43      23.5
  pro-a\1      "   136   62   46     3    11.58      24.5
               "   162   62   61     9     5.61      37.0   32.5
               "   197   87   65    11     7.11      36.5
   ra-e        "    79   44   35     1    13.33      15.5   25.0
               "   142   41   53     5     4.56      34.5
  sub-a\1      "   185   82   65     5    10.71      27.0   24.0
               "    92   39   26     1     6.73      21.0
  suf-d\1      "   158   57   77    14     3.84      40.5
               "   223  107   90     5    26.65      22.5   31.5
               "   153   66   66     7    11.26      31.0
va^virg^-dl    "    47   25   18     0     7.01       0      0
               "    66   22   23     0     5.67       0
     -l\1      "   184   69   53     5     8.05      31.0   31.0

</pre>
Seeds of mutants and other relevant lines have been sent to the cooperators
for the respective chromosomes.<p>

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