Linkage of nd, ag, and H.

Chiscon, J. A.

The following data were obtained from F2 segregations of a cross of nd; H to the 363 stock (c; h; ag, and l\1) of Dr. A. B. Burdick. Plants having few or no trichomes above the cotyledonary node were classified as hairless.

a\1   a\2   + +   a\1   a\2   a\1\a\2   Phase   % C.O.

ag    h     204   45    50       30       C     36.50±3.47
nd    ag    179   75    70        5       R     26.65±5.06
nd    h     169   80    80        0 (1)   R     11.30±5.42
The sequence of these genes on chromosome 10 appears to be H nd ag, with nd situated in close proximity to l\2. The data of A. B. Burdick (TGC-8:10), showing 10% recombination of nd with H. and the above data, showing 26.65% recombination of nd with ag, fits the accepted map distance of 46 units between H and ag reasonably well. On this basis, nd could possibly be located to the left of l\2 at approximately position 61 on chromosome 10.

Additional F2 segregation data for the above cross are as follows:

a\1   a\2   + +   a\1   a\2   a\1\a\2   Phase    % C.O.

c     h     189   60    56       24       C      45.81±3.95
l\1   h     184   65    53       27       C      44.90±3.90
nd    l\1   181   56    68       24       R      51.85±4.04
nd    c     179   66    70       14       R      41.41±4.51
c     ag    190   64    55       20       C      48.92±4.09
c     l\1   184   53    61       31       C      42.01±3.75
ag    l\1   183   54    71       21       C      49.97±4.13