Results and Discussion
The integration and expression of marker genes gus and nptII in transgenic potato were
demonstrated by activities of marker enzymes GUS and NPTII (Table 1).
Table 1. Activities of GUS and NPTII in nontransformed (control) and transgenic potato cv.
Borodyanski
Variant GUS NPTII
(imp/s/mg-equiv.of enzyme) (chlorophyll content, mg/ml of acetone
extract)
Nontransformed 0.13±0.04 103.85±4.65
Transgenic 0.43±0.01 267.05±14.35
The influence of vector A. tumefaciens EHA105 was estimated in model experiments in
which tubers infected with A. tumefaciens EHA105 were placed in a keramzit hydroponic bath and
grown for 3 months. The prolonged life of transgenic potato with the gene ugt was noted, in
comparison to potato transformed with A.t. EHA1005, but the most dramatic differences were in
sizes of developed leaves from both types of plants (Table 2).
Table 2. The comparison of leaves of potato transformed with vector A. tumefaciens EHA105 and transgenic
potato with the ugt transgene grown in a keramzit bath
Variant Mass of 1 leaf (g) Mean leaf area („ƒm2)
Transformed with A.t.EHA105 3,93±1,01 163,16±26,32
Transgenic with the gene ugt 12,76±1,38 465,26±26,88
From the data presented in Table 2, it was clear that the mass and leaf area were 3.2 and 2.9 times larger,
respectively, in transgenic potato with ugt than in transformed with blank vectorA.t. EHA105.
The increase of growth of plants transformed with the gene ugt was observed according to
morphometric analyses (Table 3).
Table 3. Morphometric analyses of potato plants at the age of buds formation growing in field
beds
Variant Height Stems Mean leaf area Leaves number
(„ƒ
m) number
(„ƒm2)
per plant
Nontransformed 36.9±6.5 2.5±0.8 277.7±81.1 21.0±5.3
Transgenic 44.7±5.8 2.8±0.6 348.4±32.6 29.2±6.6
The activity of target enzyme UDPG-transferase, free and alkali-labile IAA contents are
shown in Table 4.
Table 4. Activity of UDPG-transferase, free and alkali-labile IAA contents in potato plants cv.
Borodyanski
Variant UDPG-transferase Free IAA Alkali-labile IAA a
(nmol /mg of protein/h) (pmol/g.f.w.) ( pmol/ g.f.w. )
Nontransformed 259.6 5.1±0.6 0.0
Transgenic 1670.2 8.2±3.1 22.8±0.0
a
IAA was liberated upon 1N NaOH hydrolysis during 1 h at room temperature.
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