Figure 1. PCR analysis (A, B, C and E) and Southern blot hybridization (D) of
nontransformed and transgenic tomato T1 cv.Ventura.
A - upper gel: 1,2,3 and 4 – DNA from leaves of 4 individual transgenic tomato T1 amplified
with primers to the fragment of 234 bp from 567 to 801 nucleotides. 5 – Amplification on
plasmid DNA pBluescript with cloned ugt.
lower gel: 1,2,3 and 4 - DNA from leaves of 4 individual nontransformed tomato
amplified with the same primers. DNA standard – 100 bp liner.
B - PCR with DNA isolated from axillary sprouts of individual transgenic tomato.
C - PCR with DNA isolated from shoot (1), flower (2), 1st fruit (3), bud (4) and 2nd fruit (5).
D - Southern blot hybridization of genomic DNA restricted with EcoR1 from nontransformed
shoot (1) and root (2) of tomato, from transgenic shoot (3) and root (4) of T1 tomato. The
probe was prepared with EcoR1 restrict of insert of the gene ugt from pBluescript labeled
with 32P-CTP.
E - PCR with primers to the gene acb;
-upper gel: 1-7 - DNA from leaves of individual transgenic plants with primers to the
whole sequence of the gene acb 290 bp;
-lower gel: 1-7 - DNA from leaves of individual nontransformed plants with the
same primers.
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