Table 6. The effect of mannitol on the root formation of tomato seedlings growing in vitro on MS
salt medium
Variant
Root number Total
root
length
(mm)
Nontransformed
15.3±2.6
333.7±101.5
Transgenic
41.5±14.5
641.0±245.0
+ 0.05 „M mannitol
Nontransformed
10.5±1.5
134.5±7.5
Transgenic
27.0±7.0
263.0±59.0
+ 0.1 „M mannitol
Nontransformed
3.0±0
23.5±5.5
Transgenic
20.5±10.5
108.0±61.0
+ 0.25 „M mannitol
Nontransformed
0±0
0±0
Transgenic
41.3±14.9
216.8±101.0
UDPG-transferase in its native environment in developing corn endosperm is stable working
under high osmotic pressure created by the increased sugar content. Perhaps this property of the
target enzyme, of the transgenic T1 tomato allowed for better survival at high osmotic conditions
and drought. ACBP might be stabilized the membranes, renewing the delivering of acyl CoA esters
docking them close to fatty acid synthetase complexes.
In conclusion, it might be deduced that T1 generation of transgenic tomato with the ugt and
acb transgenes were valuable from economical point of view because of higher productivity and
sustenance to environmental conditions.
Literature cited:
Guidotti, A., Forchetti, C.M., Corda, M.G., Konkel, D., Bennett, C.D., and E. Costa. 1983. Isolation,
characterization, and purification to homogeneity of an endogenous polypeptide with
antagonistic action on benzodiazepine receptors. Proc.Natl.Acad.Sci. 80: 3531-3535.
Kolmer, M., Roos, C., Tirronen, M., Myohanen, S., and H. Alho. 1994. Tissue-specific expression of
the diazepam-binding inhibitor in Drosophila melanogaster : cloning, structure, and localization
of the gene. Molecular and Cell. Biol. 14: 6983-6995.
Murashige, T. and F. Skoog. 1962. A revised medium for rapid growth and bio-assays with tobacco
tissue cultures. Physiol. Plant. 15:473-497.
Rekoslavskaya, N.I., Salyaev, R.K., Mapelli, S., Truchin, A.A., and L.V. Gamanetz. 2002. A rise in
productivity of transgenic tomato (Lycopersicon esculentum Mill.) by transfer of the gene iaglu
from corn. TGC Report 52: 27-30.
Rekoslavskaya, N.I., Zhukova, V.M., Chekanova, E.G., Salyaev, R.K., Mapelli, S., and L.V.
Gamanetz. 1999. Auxin status of transformed Solanum plants in relation to their tolerance to
2.4-D and productivity. Russian J.Plant Physiol. 46: 609-619.
Salyaev, R.K., Rekoslavskaya, N.I., Chepinoga, A.V., Vysotskaya, E.F., Kuznetsova, E.V., Mapelli,
S., Zhukova, V.M. and N.N. Sadochina. 1999. Creation of woody plants for the Lake Baikal
region possessing the high energy of growth and increased sustenance to damage factors.
Siberian Ecological J. 6: 605-611.
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