The study of T1 generation of transgenic tomato (Lycopersicon esculentum Mill.) with
introduced genes of ugt/iaglu from Zea mays L. and acb from Arabidopsis thaliana L.
1Rekoslavskaya N.I., 1Salyaev R.K., 2Mapelli S., 1Truchin A.A. , Pacovski R.3
1Siberian Institute of Plant Physiology and Biochemistry, Siberian Branch of RAS, PO Box 1243,
Irkutsk, Russia, e-mail: phytolab@sifibr.irk.ru
2Istituto Biologia Biotecnologia Agraria, C.N.R., via Bassini 15, Milan, Italy, e-mail
mapo@ibv.mi.cnr.it
3School of Agriculture, University of Sao Paulo, 13400-900 Piracicaba, S.P. Brazil
Introduction
The gene ugt/iaglu was isolated from cDNA of seedlings of Zea mays L. (hybrid Silver Queen) and
used for transformation of Solanum species and poplar (Rekoslavskaya et al., 1999;Salyaev et al.,
1999). This gene encodes UDPG-transferase (IAA-glucose synthase) which accomodates the
content of indole acetic acid (IAA) to physiological levels in developing corn endosperm. Due to this
alkali labile, stored indolic compound, the pool of bound IAA is created in corn endosperm. After
hydrolysis released free IAA activates both division and enlargement of cells and facilitates the
growth of seedlings during germination and heterotrophic growth before protruding the earth and
beginning of photosynthesis.
UDPG-transferase used several compounds as a substrate, for example, 2,4-
dichlorophenoxyacetic acid (2,4-D). The treatment with 50 – 100 mg/l of 2,4-D of transgenic plants
of Solanum (Solanum demissum L., Solanum tuberosum L.) with introduced ugt/iaglu gene did not
reveal any herbicide action but stimulated their growth, root formation, flowering and increased
productivity (Rekoslavskaya et al. 1999).
The gene acb and its expressed protein ACBP (acyl-binding protein) were mostly studied on
animals as a diasepam binding inhibitor (DBI) which was isolated from rat brain. At first it was found
that DBI modulated the activity of neurotransmitters such as ƒÁ
amino butyric acid replacing them
from receptor cites (Guidotti et al.,1983). Later it was found that the polypeptide of 10 kD ACBP was
able to participate in the synthesis acyl-Co A esters and in its transport to microsomes and
lyposomes in oder to donate the transportable acyl CoAs for the ƒÀ
-oxidation for the synthesis of
glycerolipids (Kolmer et al., 1994). Therefore ACBP might be used for stabilizing of membrane
structures in cells. The function of ACBP in plants was unknown.
The cumulative effect of two transgenes ugt/iaglu and acb was found in transgenic poplar
(Populus tremula L.) (Salyaev et al., 1999). Transgenic poplar plants acclimatized easier and their
growth was faster if both transgenes were present when poplar plants were transfered from in vitro
conditions to open air. It was supposed that there was a synergic action of these transgenes in
growth promotion, cell division activity and sustenance to environmental conditions.
The goal of this work was to introduce the genes ugt(iaglu) and acb into tomato plants
(Lycopersicon esculentum Mill. cv. Ventura) and to evaluate their positive effect on transgenic
plants in harboring new features useful in agriculture.
Materials and Methods
The obtaining of transgenic plants of tomato cv. Ventura was described earlier
(Rekoslavskaya et al 1999). For transformation of plants a transconjugant of triparental mating of
Agrobacterium tumefaciens 699 (chromosomal base C58)
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