and leaf shape, size and color. Several traits were measured on a subset of the lines to
demonstrate phenotypic variability: total phenolics, pollen fertility, and fruit size (Table 1).
The accession used for development of the L. pimpinellifolium inbred backcross lines (IBLs)
was LA1589 (Doganlar et al. 2002), collected from the Viru valley in Department La Libertad, Peru
(8º23’24” S, 78º44’24” W). LA1269 was collected from Pisiquillo in Department Lima, Peru
(11º28’30” S, 77º6’30” W). The substantial physical distance between these collection sites, over
380 km, and evidence of genetic variation correlated with geographic location (Rick et al. 1977,
Caicedo and Schaal 2004), suggests the LA1269 and LA1589 populations might be genetically
distinct. If so, the LA1269 RIL population and the LA1589 IBL population might be complementary,
and present some interesting opportunities to compare experimental results and contrast allelic
contributions to phenotypes using both populations. The genotype data, linkage map, and seed of
the lines described here is available by contacting the first author.
This work was supported by a grant from the California Tomato Commission. We would like
to thank Roger Chetelat for helpful suggestions and generous assistance.
Table 1. Phenotypic variation for three selected traits. Phenolics measured as Quercetin
equivalent (Q Eq) in umol/gram fresh weight; Fruit size is average weight in grams for at least 20
fruit; Pollen stainability is the percentage of stainable pollen grains observed for at least 4
flowers/genotype. P value for ANOVA by genotype for at least 18 randomly selected RILs.
Trait
L. esculentum value RIL range RIL average
p value
Phenolics (Q Eq)
1.1
0.5 – 1.6
0.95
p < 0.000
Fruit size (grams)
218
1.0 – 11.9
5.8
p < 0.008
Pollen stainability (%)
81
22 - 96
76
p < 0.010
No navigation control above? Click here!
