Additional analyses of the localization of gene Ora
Avdeyev, Y.I. and Scherbinin, B.M.
Vegetable Crops Selection Laboratory. All-Russian Scientific Research Institute of Irrigated Vegetable and Melon
Growing. P.O. Box 93, Astrakhan, 414000, Russia.
In a previous publication (Avdeyev and Scherbinin, 1980) we reported data suggesting the linkage of
genes Ora and j-1+ . But the frequency of recombination between genes was not counted. Such calculations done
according to accepted method (Orlova, 1991) are presented in Table 1.
Frequency of crossing-over between gene Ora and j-1 is 0.9±0.6% in experiment 1 and 1.6±0.4% in
experiment 2. Both results are similar, but in the view of a higher accuracy of experiment 2 the distance of 2%
between genes j-1+ and Ora is taken. Taking into consideration the results of the previous recombination study
between genes Ora and j-2 (Avdeyev and Scherbinin, 1982) and their lower accuracy than in experiment 2 (Table
1), we suggest that Ora is on chromosome 11 in position 30, and not in position 33, as was proposed earlier.
Table 1. Phenotypic segregations in F2 cross combinations between Ora j+ and ora j-1
Our use of the marker mutants ele, wv, tab for localization of Ora have failed because of weak viability of
the mutant plants in the hot Astrakhan conditions in which experiments with Orobanche aegyptiaca Pers. were
carried out.
Literature Cited:
Avdeyev, Y. I., Scherbinin, B. M. 1980. TGC Report 30, r.18.
Orlova, N.N. 1991. Genetic analysis. B., r. 316 Moskow.
Avdeyev, Y.I., Scherbinin, B.M. 1982. TGC Report, 32, p. 18.
High susceptibility of fruit to Colletotrichum phomoides (Sacs.) Chest in gs/gs lines.
Avdeyev Y.I., Scherbinin B.M.
Vegetable Crops selection Laboratory. All-Russian Scientific Research Institute of Irrigated Vegetable and Melon
Growing. P.O. Box 93, Astrakhan, 414000, Russia.
It's known that gene gs is localized in chromosome 7 and causes green stripe in the epidermis of unripe
fruit and golden in ripe fruit (Rick et al., 1987). From crossing of small-fruit variety Tigerella with VF-145-F5 had
been selected a middle-term ripening line CL-F4-gs/gs with fruits by weight of 60-80 g. This was used for
hybridization with red colour fruit varieties Illinka, Volgogradsky 5/95, Richansky, Moriana, and crimson fruit
varieties Shtambovi Slivovidny and Don Huan. Studies of 147 F3 - F6 lines, selected for homozygosity of gs shows
that all were susceptible to infection of Colletotrichum phomoides (Sacc.) Chest. During the late autumnal moist
period the fruits of all lines gs/gs had clear symptoms of illness - black wet spots.
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