Popvriend Company and Peto 86 from Petoseed Company, showed high incidence of the disease, which
indicates that the imported varieties are playing a great role in the distribution of the virus.
To free the contaminated seed from seed-borne bacteria, nine treatments plus phosphate Buffer Saline
(PBS) as a negative control (table 1) were applied following the technique described by Duffe et al. (1989). To
evaluate the efficiency of these treatments, the Ouchterlony double diffusion test was used. The best results were
obtained when the contaminated seeds were extracted by fermentation and then treated with Hcl and Na-
hypochlorite (table 1). In treatments were seeds were extracted with acetic acid (No. 3 and No. 5), the three
bacteria were completely eliminated but germination of the seeds was greatly affected. Extraction of seeds with
pectinase enzyme only, has no effect in controlling the bacteria (Treatment No.7). In general, these treatments are
most effective against X. campestris pv. vesicatoria, moderate in C. michiganensis subsp michiganensis and less
effective in case of P. syringae pv. tomato.
Literature cited:
Duffe, P.H., et al. (1989). Revues Horticole No. 298: 59-63
LelIiott, R. A., and Stead De (1987). Methods for the diagnosis of bacterial diseases of plants listed. Blackwell
Scientific Publications.
Sat, M.C., et al (1989). India Rev. Plant Patho. 1990.
Silva, Mic-da, et al (19911. Rev. Plant Path. 1994.
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