* Evaluation of foliar symptoms on a scale of 0 to 4, where 0= no symptoms, 1= slight symptoms, 2= moderate
symptoms, 3= severe symptoms, and 4= very severe symptoms, including severe stunting. DPI: days post
inoculation, C: cage-inoculation, M: mass-inoculation.
** Evaluation of the effect on flowering and fruit set on a scale of 0 to 4, where 0= no effect, 1= slight flower
shedding, 2= slight flower abortion and shedding. Fruit-set lightly reduced, 3= moderate flower abortion and
shedding. Fruit-set moderately reduced, and 4= severe flower abortion and shedding. Fruit-set severely reduced.
Resistance reactions of accessions of L. peruvianum and L. chilense to spanish isolates of TSWV
RoselIo, S., Diez, M.J., Rambla, J.L. and Nuez, F.
Dept. Biotecnologia-Genetica. Universidad Politecnica de Valencia. 46022 Valencia. Espana.
Some accessions of Lycopersicon peruvianum, L. chilense and materials derived from L, chilense were
inoculated mechanically with two spanish isolates of TSWV. These two isolates were selected for their high
virulence and different biological and biochemical characteristics (Abad, 1996). The accessions tested were
chosen by their high level of resistance to TSWV in field conditions (Table 1). Inoculations were conducted in a
climatic chamber with the following environmental conditions. 25°/18°C of temperature (day/night), 60-65% / 95-
100% of RH (day/night), 65-85 µ mol m-2 s-1 of irradiance and photoperiod of 14h of light. Plants were inoculated at
4-leaf stage. A second treatment was repeated a week after the first inoculation. The tomato line NE-1 was used
as susceptible control . Plants remained in the climatic chamber for two months. Plants were scored for
appearance and severity of symptoms. ELISA assay was performed each 10-15 days. Visual symptoms and
results of ELISA were used as a criteria
No navigation control above? Click here!
