Stability of Frl gene
Moretti, A. 1,
Troulet, C.2, Laterrot, H.1
1 INRA - BP 94 - 84143 Montfavet Cedex France
2
LRPV - BP 94 - 84143 Montfavet Cedex France
Fusarium oxysporum f. sp. radicis lycopersici (= FORL) is an important problem for the tomato especially
in soilless cultures and sometimes in sterilized sandy soil. The FORL resistance issued from Lycopersicon
peruvianum is controlled by the dominant gene Fri. The linkage of Frl with TMV resistance (alleles Tm-2 and Tm-
22) provides a fast solution to obtain FORL resistant F1 hybrids. Therefore, the Frl gene was used without a
previous study of its efficiency against the pathogen variability.
To estimate the Fri stability we collected FORL isolates in various conditions
- 119 FORL isolates in different French regions.
- 27 isolates from 8 countries : Spain (13), Great Britain (4), Switzerland (4), Netherlands (2), Israel,
Italy, Morocco, Cameroon (1 isolate).
The 146 isolates were studied in climatic chamber by artificial inoculation of 10 plants of 4 near isogenic
lines in the Moneymaker type (Laterrot TGC Report No 46 p. 35)
- Mossol and Motelle, lines without Frl gene
- Momor and Mogeor, lines with Frl gene.
The inoculation was realized by dipping roots and hypocotyl of 18 days old plants during 5 minutes in a
conidia suspension with a concentration of 107 conidia per ml.
Five of the 146 isolates were able to affect the 4 genotypes. Four of these were isolated from FORL
susceptible varieties in France (3 isolates) and in Morocco (1 isolate), and one isolated in France from a resistant
F1, hybrid carrying the genotype FrI/Frl+
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