Location of Dwarf on the Classical and RFLP maps
Bishop, G.2, Thomas, C.1 and Jones, J.1
'The Sainsbury Laboratory, John Innes Centre, Norwich, NR4 7UH, U K.; 2Current address, Plant Hormone
Function Group, Frontier Research Program, RIKEN, 2-1 Hirosawa, Wako-shi, Saitama, 351-01, JAPAN. (e-mail
bishopg@postman.riken.go.jp)
The isolation of the tomato Dwarf gene (Bishop et al. 1996), provided a probe for mapping Dwarf on the
tomato RFLP map of tomato. This was carried out using an EcoR I digest of DNA made from an L. esculentum x
L. pennellii F2 mapping population (Tanksley et al., 1992). These data, in conjunction with RFLP data provided by
Steve Tanksley and obtained from the SolGenes database, were used to place Dwarf 4% (recombination) North of
marker TG492, using the MAPMAKER program. The T-DNA of transformant 851Q mapped previously to
chromosome 2 (Thomas et al. 1994) was placed 5.2 % North of Dwarf and 5.4% South of CT229C. Genetic
linkage data between dwarf, 851Q T-DNA and other chromosome 2 mutations were also generated.
Linkage analysis of T-DNA 851Q (Kanamycin Resistance) to wv, aa, and d.
F2 progeny phenotypes generated from the F1 of 851Q X LA1700 (wv-aa-d). + = wild type, wv = white virescent,
aa = anthocyanin absent, d = dwarf.
Linkage calculated using maximum likelihood method (Mather, 1951) with the total for all sets of data.
aa-d
15.8% ±1.5%
851 Q-wv
20.0% ±1.7%
wv-aa
4.1% ±0.8%
851 Q-aa
15.4% ±1.5%
wv-d
20.1% ±1.7%
851Q-d
1.0% ±0.4%
Literature Cited:
Bishop G.J., Harrison K. and Jones, J. D. G. (1996). Plant Cell 8, 959-969
Mather, K. (1951). The measurement of Linkage in Heredity. (London: Methuen.)
Tanksley, S.D., Ganal, M.W., Prince, J.P., de Vicente, M.C., Bonierbale, M.W., Broun, P., Fulton, T.M.,
Giovannoni, J.J., Grandillo, S., Martin, G.B., Messeguer, R., Miller, J.C., Miller, L., Paterson, A.H., Pineda,
0., Roder, M.S., Wing, R.A., Wu, W., and Young, N.D. (1992). Genetics 132,1141-1160.
Thomas, C.M., Jones, D.A., English, J.J., Carroll, B.J., Bennetzen, J.L., Harrison, K.A., Burbidge, A., Bishop, G.J.,
and Jones, J.D.G. (1994). Mol. Gen. Genet. 242, 573-585.
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