Observation
upon
the
comparative
use
of
two
isozyme
loci
in
F,
tomato
hybrids
Vodenicharova,
M
.
S
.
,
Stoilova,
Ts
.
B
.
,
Markova,
M
.
D
.
and
Cholakova,
N
.
I
.
Inst
.
of
Genetics,
Bulgarian
Academy
of
Sciences,
Sofia
-
13,
Bulgaria
In
our
previous
work
it
was
estimated
that
some
isoenzyme
molecular
forms
of
the
enzymes
alchohol
dexydrogenase
and
esterase
may
be
used
as
genetic
markers
for
tomato
seed
hybridity
(TGC
Report,
1993,
No
.
43,
p
.
50)
.
These
isoenzyme
molecular
forms
later
were
sighted
as
locus
Adh
-
1
and
locus
Est
-
1
(Compt
.
Rend
.
Acad
.
Bulg
.
Sci
.
,
1993,
No
.
46,
pp
.
89,
97)
.
In
this
study
we
have
compared
the
application
of
the
two
isozyme
loci
for
F,
tomato
hybridity
determination
.
2113
individual
seeds
of
tomato
(
L
.
esculentum
Mill
.
)
hybrids
and
their
parental
lines,
taken
from
different
lots
and
experimental
stations
in
Bulgaria,
were
analyzed
(Table
1)
.
The
enzyme
extractions,
the
electrophoretic
division
on
polyacrilamide
gel
and
the
isozyme
visualization
were
carried
out
by
our
previous
work
.
The
two
loci
for
tomato
hybridity
identification
were
used
only
in
Kristi
and
Standard
59
cultivars
.
These
tomato
hybrids
were
distinguished
for
their
mother
line
which
had
the
gene
of
make
sterility:
ms
-
35
.
The
results
showed
complete
comparison
of
the
loci
Est
-
1
and
Adh
-
1
as
markers
of
F
1
hybridity
.
In
the
other
tomato
hybrids
that
we
have
investigated,
we
were
not
able
to
use
the
locus
Adh
-
1
because
of
lack
of
alternative
isozymes
in
the
parental
lines
.
Therefore
this
locus
wasn't
applicable
to
prove
F,
hybridityin
all
tomato
cultivars,
i
.
e
.
its
application
was
limited
.
As
hybrid
marker
for
the
other
tomato
varieties
we
used
only
locus
Est
-
1
.
There
was
no
difference
between
the
locus
Est
-
1
expressions
for
the
proving
of
F
1
hybridity
in
combinations
both
from
different
regions
of
the
country
and
with
different
origin
.
The
facts
of
great
importance
were
the
quicker
esterase
isozymes
visualization
and
the
cheaper
chemicals
for
if
.
The
data
of
this
research
indicate
some
universality
of
locus
Est
-
1
for
proving
F
1
tomato
hybridity
.
Literature
cited:
Stoilova,
Ts
.
B
.
,
M
.
S
.
Vodenicharova,
M
.
D
.
Markova
.
1993
.
Compt
.
Rend
.
Acad
.
Bulg
.
Sci
.
46:89
-
91
Vodenicharova,
M
.
S
.
,
Ts
.
B
.
Stoilova,
M
.
D
.
Markova
.
1993
.
Compt
.
Rend
.
Acad
.
Bulg
.
Sci
.
46:97
-
99
Vodenicharova,
M
.
S
.
,
Ts
.
B
.
Stoilova,
N
.
l
_
Cholakova,
M
.
D
.
Markova,
M
.
V
.
Ancheva
.
1993
.
TGC
Report
43:50
-
51
.
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