Table 1. Results of filter hybridization of L. peruvianum genomic DNA to the S7 and S6
probes conducted under moderate (68°C) stringency. Accessions are listed in approximately ascending south
latitude.
Literature Cited:
Bernatzky, R., M.A. Anderson, A.E. Clarke. 1988. Molecular genetics of self-incompatibility in flowering plants. Dev.
Genet. 9: 1-12.
loerger, T.R., A.G. Clark, T-H. Kao. 1990. Polymorphism at the self-incompatibility locus in Solanaceae predates
speciation. Proc. Nat. Acad. Sci. 87: 9732-9735.
Tsai, D-S, H-S Lee, L.C.Post, K.M. Kreilling, T-H Kao 1992. Sequence of an S-protein of Lycopersicon peruvianum
and comparison with other solanaceous S-proteins. Sex. Plant Reprod. 5: 256-263.
Rivers, B.A., R. Bernatzky, S.J. Robinson, W. Jahnen-Dechent 1993. Molecular diversity at the self-incompatibility
locus is a salient feature in natural populations of wild tomato (Lycopersicon peruvianum). Mol. Gen. Genet.
238: 419-427.
Mapping of tomato genes induced in a compatible root-knot nematode interaction
Llharska1,T. , Van der Eycken2, W. , Gheysen2, G. and Zabel1, P.
1
Wageningen Agricultural University, Dept. of Molecular Biology, Dreijenlaan 3, 6703HA Wageningen, The
Netherlands, 2University of Ghent, Lab.Genetics, K. L. Ledeganckstraat 35, B-9000 Ghent, Belgium
In case of a compatible interaction, root-knot nematodes prompt the development of complex feeding sites
(galls containing giant cells) in the root system by altering the expression of plant genes. Tomato (Lycopersicon
esculentum cv. Marmande) genes that are either induced or repressed upon nematode infection (referred to as
Lemmi genes) have been isolated from a cDNA library made from root galls induced by Meloidogyne incognita race
1( Van der Eycken et al. 1992a, 1992b). Three Lemmi genes (Lemmi 2, Lemmi 9 and Lemmi 10 ) that are highly
expressed in galls, particularly in the giant cells, have been chosen for further studies (van der Eycken at al., in
prep.). When looking for possible functions in giant cells, one may compare the map position of Lemmi genes with
the position of genes already mapped (Tanksley et al., 1992). Mapping nematode induced-genes would also
provide information as to their distribution among the tomato chromosomes and, thus, supplement the genetic
linkage map of tomato.
We have mapped Lemmi genes 2, 9 and 10 by RFLP linkage analysis using an F2 mapping population (84
plants) derived from a cross L. esculentum cv. Allround x L. pennellii LA716 (Odinot et al. 1992) that has been
characterized with 38 RFLP markers
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