Kesicki, E. The use of immunosuppressors in interspecific hybridization of tomato.
Bates and Deyoe (1973, Economic Botany 27) postulated the use of chemicals which suppress
immunological reactions in animal tissue for breaking the incompatibility barriers in plants. The use of such
chemicals was successful in cereals (Bates et al. 1974, Cereal Science Today 19). Present study indicates that
immunosuppressors also give positive results in tomato, at least in these analyzed combinations: L. esculentum X L.
hirsutum and L. esculentum X L. peruvianum.
L. esculentum cv. Potentate, L. hirsutum PI 308182 and L. peruvianum PI 126435 stocks were used.
Flowers of L. esculentum were emasculated and pollinated with the other species before treatment. The treatments
were carried out once a day for 14 days after pollination.
The best results were obtained with Cuprenil in 0.05% solution with the addition of DMSO. This treatment
distinctly increased the mean number of hybrid seeds per fruit, the percent of seed germination, and the mean
number of hybrid plants per fruit in the test combination L. esculentum X L. hirsutum (see table).
Applications of Cuprenil were also successful in crosses of L. esculentum and L. peruvianum. After
treatment of plants with Cuprenil, 43 F1 hybrids and many seeds of BC1 to L. esculentum were obtained.
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