Other factors
Stem explants: 1) Floral branches. The stem segments excised from below the floral branches gave rapid
and direct regeneration, while those collected from more basal parts of plants gave no satisfactory results. 2)
Epidermis. When compared to the stem explants with intact epidermis, those devoid of epidermal tissue did not form
callus or gave very poor callus after long periods of culture; in such cultures the degree of polyploidization was high.
Anther culture: 1) Stage of pollen development. The culture of anthers in meiotic stage gave no haploid
plants. The mononucleate-mitosis gave embryoid formation in the hybrid, and the meiotic stage gave regeneration
to diploids and tetraploids in L. peruvianum. 2) Other conditions of culture. The soaking of flower buds in 2,4-D prior
to anther culture, close proximity of anthers on the medium and light during culture increased the development and
frequency of embryogenic anthers (Cappadocia et al. unpubl.).
Tanksley, S. D. Adh and Pgm phenotypes.
Previous research has demonstrated that two genes, one coding for alcohol dehydrogenase and the other
for phosphoglucomutase, are tightly linked (Tanksley, TGC 28:20). Since that first report, it has been possible, using
allele dosage effects in trisomics, to assign both of these genes, designated Adh-1 and Pgm-2, to the long arm of
chromosome 4. (Details of this research are being reported elsewhere). Assaying for phosphoglucomutase activity
in various tissues and organs of developing tomato plants has revealed another gene coding for isozymes
expressing activity in chlorophyllous tissue. Since these isozymes band more anodally than any of the allozymes of
the pgm locus, the gene is given the symbol Pgm-1.
The purpose of this report is to describe the banding phenotypes of the known alleles for these three loci.
The following diagram is similar in format to those of earlier reports on Prx (Rick and Fobes, TGC 26:15), Aps, Est,
and Got (Fobes and Rick, TGC 26:7) and should be self explanatory. Some alleles have been omitted since lack of
adequate testing prevents unambiguous discrimination from existing alleles, and accounts for gaps in the
consecutive order of the list. All alleles described here, however, are confirmed to be mutually exclusive.
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