5.2%  of  the  seeds  originate  from  self-pollination  of  the  female  parent.  (We  obtained  4.5% selfing  in  1976  in  a  similar  experiment  but  without  fruit  by  fruit  extraction  of  the  seeds).  The best part of this percentage was obtained from a few fruits which yielded 100% seeds issuing from self-pollination. The frequency with which these fruits were obtained at certain times during the flowering period was high. However,  it  is  impossible  to  conclude  from  our  data  to  what  extent  dehiscence  of  the stamens  of  ps-2  flowers  is  encouraged  by  emasculation  and  to  what  extent  it  occurs  naturally  and independently of the latter operation (due to wind, dry weather etc.). Rao, R. N., and Panuganti N. Rao A highly sterile unstable tetraploid of tomato. Among the colchicine induced tetraploids of tomato var. Marglobe, one showed low multi-valent formation at diakinesis (average fre- quency of 1.1 quadrivalents per cell), but meiotic abnormalities like multipolar spindles (1-5 with varying number of chromosomes in each) at metaphase I, laggards at  anaphase  I  and  several  groups  (up  to  4)  of  chromosomes  at  telophase  I  etc.  were  found  in most  of  the  cells.  Pollen  sterility  was  very  high  (85.00%).  Whether  similar  irregularities  also occurred on the female side leading to sterility could not be ascertained as the plant was growing and   flowering   in   the   field   in   April-May   with   rising   summer   temperatures   resulting   in   flower abscission  despite  pollinations  with  normal  tetraploids.  In  the  diploids  and  other  tetraploids  also growing in the same period, fruiting was found to be scanty, though meiosis in PMC's was normal with  good  pollen  fertility.  The  type  of  meiotic  abnormalities  found  in  the  sterile  tetraploid  is apparently  an  indication  of  its  instability  and  tendency  towards  breakdown  into  lower  levels  of ploidy. Rao,  R.  N.,  and  Panaganti  N.  Rao  A  purple stigma variant in Lycopersicon glandulosum. One plant in a population of L. glandulosum (seeds obtained  from  Plant  Introduction  Centre,  Indian Agricultural  Research Institute, New Delhi; Acc. No. EC 66003, Ex U.S.A.) raised through open pollinated seed for three generations here in Botany Experimental Field Station showed purple coloration of the stigma. This is evident even at the bud stage and persists till withering of the stigma. Other characters associated with this color variation are the prominent exsertion of the style (largely due to the down-curling of the sterile tip of the staminal cone), shorter staminal cone, and reduction in diameter of the open flowers. Compared to normal sibling plants, the overall length of the style is actually shorter but it is more exposed only due to the shorter anther column. The magnitude of differences in all these characters between the variant individual and the normal siblings is given in table below. The nature of association of these characters with coloration of the stigma is under investigation.  

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